1sxm

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[[Image:1sxm.gif|left|200px]]
 
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{{Structure
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==SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)==
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|PDB= 1sxm |SIZE=350|CAPTION= <scene name='initialview01'>1sxm</scene>
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<StructureSection load='1sxm' size='340' side='right'caption='[[1sxm]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>
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<table><tr><td colspan='2'>[[1sxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXM FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxm OCA], [https://pdbe.org/1sxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxm RCSB], [https://www.ebi.ac.uk/pdbsum/1sxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxm ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxm OCA], [http://www.ebi.ac.uk/pdbsum/1sxm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sxm RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/KAX21_CENNO KAX21_CENNO] Blocks voltage-gated non-inactivating potassium channels and unblocks inactivating potassium channels blocked by alpha-dendrotoxin in synaptosomes. Also displaces the alpha-dendrotoxin homolog dendrotoxin I from its receptor on brain synaptic membranes.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxm ConSurf].
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<div style="clear:both"></div>
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'''SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)'''
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==See Also==
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*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The 3D structure of noxiustoxin, the first identified scorpion toxin acting on K+ channels, has been elucidated by NMR and molecular modeling. Thirty-nine solution structures were calculated using 572 distance and 42 dihedral restraints. The average atomic rms deviation between the refined structures and the mean structure is 0.75 A for the backbone atoms. Noxiustoxin adopts a alpha/beta scaffold constituted of a three-stranded beta-sheet (residues 2-3, 25-30, 33-38) linked to a helix (residues 10-20) through two disulfide bridges. A comparison between the 3D structure of noxiustoxin and those of other structurally and functionally related scorpion toxins (charybdotoxin, PO5-NH2, kaliotoxin) revealed a bending capacity of the helix and a variability in the relative orientations between the helix and the beta-sheet. These two features highlight the plasticity of the alpha/beta scaffold and offer a structural explanation for the capacity of the fold to accommodate an additional alanine residue in the Gly-x-Cys pattern of a previously proposed consensus sequence [Bontems et al. (1991) Science 254, 1521-1523]. Our structural data also emphasize the possibility that the beta-sheet of NTX is implicated in the capacity of NTX to recognize voltage-dependent K+ channels.
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==About this Structure==
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1SXM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXM OCA].
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==Reference==
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Determination of the three-dimensional solution structure of noxiustoxin: analysis of structural differences with related short-chain scorpion toxins., Dauplais M, Gilquin B, Possani LD, Gurrola-Briones G, Roumestand C, Menez A, Biochemistry. 1995 Dec 26;34(51):16563-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8527429 8527429]
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[[Category: Centruroides noxius]]
[[Category: Centruroides noxius]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dauplais, M.]]
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[[Category: Dauplais M]]
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[[Category: Gilquin, B.]]
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[[Category: Gilquin B]]
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[[Category: Gurrola-Briones, G.]]
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[[Category: Gurrola-Briones G]]
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[[Category: Menez, A.]]
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[[Category: Menez A]]
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[[Category: Possani, L D.]]
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[[Category: Possani LD]]
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[[Category: Roumestand, C.]]
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[[Category: Roumestand C]]
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[[Category: toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:48:21 2008''
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Current revision

SCORPION TOXIN (NOXIUSTOXIN) WITH HIGH AFFINITY FOR VOLTAGE DEPENDENT POTASSIUM CHANNEL AND LOW AFFINITY FOR CALCIUM DEPENDENT POTASSIUM CHANNEL (NMR AT 20 DEGREES, PH3.5, 39 STRUCTURES)

PDB ID 1sxm

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