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5uv0
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of (+)-Limonene Synthase from Citrus sinensis== | |
| + | <StructureSection load='5uv0' size='340' side='right'caption='[[5uv0]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5uv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_sinensis Citrus sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UV0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uv0 OCA], [https://pdbe.org/5uv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uv0 RCSB], [https://www.ebi.ac.uk/pdbsum/5uv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uv0 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RLC1_CITSI RLC1_CITSI] Catalyzes the conversion of geranyl diphosphate to (+)-(4R)-limonene (PubMed:28272875, PubMed:28272876). Produces exclusively the (+)-enantiomer (PubMed:28272875). Can use neryl diphosphate as substrate (PubMed:28272876). Has no activity with farnesyl diphosphate (PubMed:28272875).<ref>PMID:28272875</ref> <ref>PMID:28272876</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. Limonene is a cyclic monoterpene (C10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. The mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzymes that generate (+)-limonene, as well. Here we report the first cloning and biochemical characterization of a (+)-limonene synthase from navel orange (Citrus sinensis). The enzyme obeys classical Michaelis-Menten kinetics and produces exclusively the (+)-enantiomer. We have determined the crystal structure of the apoprotein in an "open" conformation at 2.3 A resolution. Comparison with the structure of (-)-limonene synthase (Mentha spicata), which is representative of a fully closed conformation (Protein Data Bank entry 2ONG ), reveals that the short H-alpha1 helix moves nearly 5 A inward upon substrate binding, and a conserved Tyr flips to point its hydroxyl group into the active site. | ||
| - | + | Functional and Structural Characterization of a (+)-Limonene Synthase from Citrus sinensis.,Morehouse BR, Kumar RP, Matos JO, Olsen SN, Entova S, Oprian DD Biochemistry. 2017 Mar 15. doi: 10.1021/acs.biochem.7b00143. PMID:28272875<ref>PMID:28272875</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5uv0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Citrus sinensis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oprian DD]] | ||
| + | [[Category: Prem Kumar R]] | ||
Current revision
Crystal Structure of (+)-Limonene Synthase from Citrus sinensis
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