5x31
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5x31 is ON HOLD until Paper Publication Authors: Fukuda, Y., Mizohata, E., Inoue, T. Description: Pseudoazurin from Alcaligenes faecalis (space gro...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Pseudoazurin from Alcaligenes faecalis (space group P65)== | |
| + | <StructureSection load='5x31' size='340' side='right'caption='[[5x31]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5x31]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X31 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x31 OCA], [https://pdbe.org/5x31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x31 RCSB], [https://www.ebi.ac.uk/pdbsum/5x31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x31 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pseudoazurin from the denitrifying bacterium Alcaligenes faecalis (AfPAz) is a blue copper protein and functions as an electron donor to copper-containing nitrite reductase (CuNIR). Conventionally, AfPAz has been crystallized using highly concentrated ammonium sulfate as a precipitant. Here, a needle-like crystal of AfPAz grown in a solution containing a macromolecular precipitant, polyethylene glycol 8000 (PEG 8000), is reported. The crystal belonged to space group P61, with unit-cell parameters a = b = 68.7, c = 94.2 A. The structure has been determined and refined at 2.6 A resolution. The asymmetric unit contained two AfPAz molecules contacting each other on negatively charged surfaces. The molecular packing of the crystal showed a right-handed double-helical arrangement of AfPAz molecules and hence of blue copper sites. This structure provides insight into the excluded-volume effect of PEG and the manner of assembly of AfPAz. | ||
| - | + | New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.,Fukuda Y, Mizohata E, Inoue T Acta Crystallogr F Struct Biol Commun. 2017 Mar 1;73(Pt 3):159-166. doi:, 10.1107/S2053230X17002631. Epub 2017 Feb 28. PMID:28291752<ref>PMID:28291752</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5x31" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| + | *[[Pseudoazurin|Pseudoazurin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Alcaligenes faecalis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Fukuda Y]] | ||
| + | [[Category: Inoue T]] | ||
| + | [[Category: Mizohata E]] | ||
Current revision
Pseudoazurin from Alcaligenes faecalis (space group P65)
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