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1t3t

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Structure of Formylglycinamide synthetase

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Retrieved from "http://52.214.119.220/wiki/index.php/1t3t"

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-[[Image:1t3t.gif|left|200px]]
- {{Structure
+==Structure of Formylglycinamide synthetase==
-|PDB= 1t3t |SIZE=350|CAPTION= <scene name='initialview01'>1t3t</scene>, resolution 1.90&Aring;
+<StructureSection load='1t3t' size='340' side='right'caption='[[1t3t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CYG:2-AMINO-4-(AMINO-3-OXO-PROPYLSULFANYLCARBONYL)-BUTYRIC+ACID'>CYG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+<table><tr><td colspan='2'>[[1t3t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T3T FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphoribosylformylglycinamidine_synthase Phosphoribosylformylglycinamidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.3 6.3.5.3] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-|GENE= PURL, STM2565 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CYG:2-AMINO-4-(AMINO-3-OXO-PROPYLSULFANYLCARBONYL)-BUTYRIC+ACID'>CYG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3t OCA], [https://pdbe.org/1t3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t3t RCSB], [https://www.ebi.ac.uk/pdbsum/1t3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t3t ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3t OCA], [http://www.ebi.ac.uk/pdbsum/1t3t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t3t RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/PUR4_SALTY PUR4_SALTY]
+== Evolutionary Conservation ==
-'''Structure of Formylglycinamide synthetase'''
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
-==Overview==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t3/1t3t_consurf.spt"</scriptWhenChecked>
-Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, P(i), and glutamate in the fourth step of the purine biosynthetic pathway. In eukaryotes and Gram-negative bacteria, FGAR-AT is encoded by the purL gene as a multidomain protein with a molecular mass of about 140 kDa. In Gram-positive bacteria and archaebacteria FGAR-AT is a complex of three proteins: PurS, PurL, and PurQ. We have determined the structure of FGAR-AT (PurL) from Salmonella typhimurium at 1.9 A resolution using X-ray crystallography. PurL is the last remaining enzyme in the purine biosynthetic pathway to have its structure determined. The structure reveals four domains: an N-terminal domain structurally homologous to a PurS dimer, a linker region, an FGAM synthetase domain homologous to an aminoimidazole ribonucleotide synthetase (PurM) dimer, and a triad glutaminase domain. The domains are intricately linked by interdomain interactions and peptide connectors. The fold common to PurM and the central region of PurL represents a superfamily for which HypE, SelD, and ThiL are predicted to be members. A structural ADP molecule was found bound to a site related to the putative active site by pseudo-2-fold symmetry and two sulfate ions were found at the putative active site. These observations and the structural similarities between PurM and StPurL were used to model the substrates FGAR and ATP in the StPurL active site. A glutamylthioester intermediate was found in the glutaminase domain at Cys1135. The N-terminal (PurS-like) domain is hypothesized to form the putative channel through which ammonia passes from the glutaminase domain to the FGAM synthetase domain.
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==About this Structure==
+  </jmolCheckbox>
-T3T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3T OCA].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t3t ConSurf].
+<div style="clear:both"></div>
-==Reference==
+__TOC__
-Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography., Anand R, Hoskins AA, Stubbe J, Ealick SE, Biochemistry. 2004 Aug 17;43(32):10328-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15301531 15301531]
+</StructureSection>
-[[Category: Phosphoribosylformylglycinamidine synthase]]
+[[Category: Large Structures]]
-[[Category: Salmonella typhimurium]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-[[Category: Single protein]]
+[[Category: Anand R]]
-[[Category: Anand, R.]]
+[[Category: Ealick SE]]
-[[Category: Ealick, S E.]]
+[[Category: Hoskin AA]]
-[[Category: Hoskin, A A.]]
+[[Category: Stubbe J]]
-[[Category: Stubbe, J.]]
-[[Category: fgam synthetase]]
-[[Category: formyl glycinamide]]
-[[Category: purl]]
-[[Category: purq]]
-[[Category: pur]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:50:53 2008''

1t3t

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Structure of Formylglycinamide synthetase

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