This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1t5e

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:38, 1 May 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1t5e.gif|left|200px]]
 
-
{{Structure
+
==The structure of MexA==
-
|PDB= 1t5e |SIZE=350|CAPTION= <scene name='initialview01'>1t5e</scene>, resolution 3.&Aring;
+
<StructureSection load='1t5e' size='340' side='right'caption='[[1t5e]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=3GR:GLYCERALDEHYDE'>3GR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+
<table><tr><td colspan='2'>[[1t5e]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T5E FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
-
|GENE= MEXA, PA0425 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3GR:GLYCERALDEHYDE'>3GR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5e OCA], [https://pdbe.org/1t5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t5e RCSB], [https://www.ebi.ac.uk/pdbsum/1t5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t5e ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t5e OCA], [http://www.ebi.ac.uk/pdbsum/1t5e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t5e RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/MEXA_PSEAE MEXA_PSEAE] The periplasmic linker component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Required for assembly of the MexA/MexB/OprM complex. Implicated in the secretion of the siderophore pyoverdine.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref> The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.<ref>PMID:8226684</ref> <ref>PMID:8540696</ref> <ref>PMID:9603892</ref>
-
 
+
== Evolutionary Conservation ==
-
'''The structure of MexA'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t5/1t5e_consurf.spt"</scriptWhenChecked>
-
Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane transporter such as the AcrB proton antiporter, an outer membrane exit duct of the TolC family, and a periplasmic protein known as the adaptor. We present the x-ray structure of the MexA adaptor from the human pathogen Pseudomonas aeruginosa. The elongated molecule contains three linearly arranged subdomains; a 47-A-long alpha-helical hairpin, a lipoyl domain, and a six-stranded beta-barrel. In the crystal, hairpins of neighboring MexA monomers pack side-by-side to form twisted arcs. We discuss the implications of the packing of molecules within the crystal. On the basis of the structure and packing, we suggest a model for the key periplasmic interaction between the outer membrane channel and the adaptor protein in the assembled drug efflux pump.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1T5E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5E OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t5e ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
== References ==
-
Structure of the periplasmic component of a bacterial drug efflux pump., Higgins MK, Bokma E, Koronakis E, Hughes C, Koronakis V, Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):9994-9. Epub 2004 Jun 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15226509 15226509]
+
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
-
[[Category: Single protein]]
+
[[Category: Bokma E]]
-
[[Category: Bokma, E.]]
+
[[Category: Higgins MK]]
-
[[Category: Higgins, M K.]]
+
[[Category: Hughes C]]
-
[[Category: Hughes, C.]]
+
[[Category: Koronakis E]]
-
[[Category: Koronakis, E.]]
+
[[Category: Koronakis V]]
-
[[Category: Koronakis, V.]]
+
-
[[Category: antibiotic efflux pump]]
+
-
[[Category: mexa]]
+
-
[[Category: periplasmic adaptor protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:51:34 2008''
+

Current revision

The structure of MexA

PDB ID 1t5e

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t5e"
Personal tools