1t6v

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[[Image:1t6v.gif|left|200px]]
 
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{{Structure
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==Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme==
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|PDB= 1t6v |SIZE=350|CAPTION= <scene name='initialview01'>1t6v</scene>, resolution 1.70&Aring;
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<StructureSection load='1t6v' size='340' side='right'caption='[[1t6v]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>
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<table><tr><td colspan='2'>[[1t6v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Ginglymostoma_cirratum Ginglymostoma cirratum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T6V FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t6v OCA], [https://pdbe.org/1t6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t6v RCSB], [https://www.ebi.ac.uk/pdbsum/1t6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t6v ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1sq2|1SQ2]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t6v OCA], [http://www.ebi.ac.uk/pdbsum/1t6v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t6v RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t6/1t6v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t6v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
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'''Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme'''
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Crystal structure of a shark single-domain antibody V region in complex with lysozyme.,Stanfield RL, Dooley H, Flajnik MF, Wilson IA Science. 2004 Sep 17;305(5691):1770-3. Epub 2004 Aug 19. PMID:15319492<ref>PMID:15319492</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1t6v" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1T6V is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Ginglymostoma_cirratum Ginglymostoma cirratum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6V OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of a shark single-domain antibody V region in complex with lysozyme., Stanfield RL, Dooley H, Flajnik MF, Wilson IA, Science. 2004 Sep 17;305(5691):1770-3. Epub 2004 Aug 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15319492 15319492]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Ginglymostoma cirratum]]
[[Category: Ginglymostoma cirratum]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Dooley H]]
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[[Category: Dooley, H.]]
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[[Category: Flajnik MF]]
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[[Category: Flajnik, M F.]]
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[[Category: Stanfield RL]]
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[[Category: Stanfield, R L.]]
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[[Category: Wilson IA]]
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[[Category: Wilson, I A.]]
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[[Category: immunoglobulin fold]]
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[[Category: protein-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:52:07 2008''
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Current revision

Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme

PDB ID 1t6v

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