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4r0t

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==Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr==
==Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr==
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<StructureSection load='4r0t' size='340' side='right' caption='[[4r0t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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<StructureSection load='4r0t' size='340' side='right'caption='[[4r0t]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4r0t]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R0T FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4r0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0T FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.603&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4r0s|4r0s]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0t OCA], [http://pdbe.org/4r0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4r0t RCSB], [http://www.ebi.ac.uk/pdbsum/4r0t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0t ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0t OCA], [https://pdbe.org/4r0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0t RCSB], [https://www.ebi.ac.uk/pdbsum/4r0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0t ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9HXC7_PSEAE Q9HXC7_PSEAE]
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Biofilms are important for cell communication and growth in most bacteria, and are responsible for a number of human clinical infections and diseases. TpbA (PA3885) is a dual specific tyrosine phosphatase (DUSP) that negatively regulates biofilm formation in the opportunistic pathogen Pseudomonas aeruginosa PAO1 by converting extracellular quorum sensing signals into internal gene cascade reactions that result in reduced biofilm formation. We have determined the three-dimensional crystal structure of wild-type TpbA from P. aeruginosa PAO1 in the phosphate-bound state and a TpbA (C132S) mutant with phosphotyrosine. Comparison between the phosphate-bound structure and the previously reported ligand-free TpbA structure reveals the extent of conformational changes that occur upon substrate binding. The largest changes occur in the functional loops that define the substrate binding site, including the PTP, general acid and alpha4-alpha5 loops. We further show that TpbA efficiently catalyzes the hydrolysis of two phosphotyrosine peptides derived from the periplasmic domain of TpbB (YfiN, PA1120), with a strong preference for dephosphorylating Tyr48 over Tyr62. This work adds to the small repertoire of DUSP structures in both the ligand-free and ligand-bound states, and provides a starting point for further study of the role of TpbA in biofilm formation.
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Structural and Biochemical Analysis of Tyrosine Phosphatase Related to Biofilm Formation A (TpbA) from the Opportunistic Pathogen Pseudomonas aeruginosa PAO1.,Xu K, Li S, Yang W, Li K, Bai Y, Xu Y, Jin J, Wang Y, Bartlam M PLoS One. 2015 Apr 24;10(4):e0124330. doi: 10.1371/journal.pone.0124330., eCollection 2015. PMID:25909591<ref>PMID:25909591</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4r0t" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Tyrosine phosphatase|Tyrosine phosphatase]]
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*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bartlam, M]]
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[[Category: Large Structures]]
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[[Category: Li, S]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Wang, Y]]
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[[Category: Bartlam M]]
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[[Category: Xu, K]]
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[[Category: Li S]]
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[[Category: Dusp fold]]
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[[Category: Wang Y]]
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[[Category: Hydrolase]]
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[[Category: Xu K]]
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[[Category: Protein tyrosine phosphatase]]
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Current revision

Crystal structure of P. aeruginosa TpbA (C132S) in complex with pTyr

PDB ID 4r0t

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