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| | ==Cryo-EM reconstruction of quasi-HPV16 complex with H16.1A Fab== | | ==Cryo-EM reconstruction of quasi-HPV16 complex with H16.1A Fab== |
| - | <StructureSection load='3j8z' size='340' side='right' caption='[[3j8z]], [[Resolution|resolution]] 14.00Å' scene=''> | + | <SX load='3j8z' size='340' side='right' viewer='molstar' caption='[[3j8z]], [[Resolution|resolution]] 14.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3j8z]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J8Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3j8z]] is a 13 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J8Z FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j8w|3j8w]], [[3j8v|3j8v]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 14Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8z OCA], [http://pdbe.org/3j8z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j8z RCSB], [http://www.ebi.ac.uk/pdbsum/3j8z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j8z OCA], [https://pdbe.org/3j8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j8z RCSB], [https://www.ebi.ac.uk/pdbsum/3j8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j8z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3j8z" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3j8z" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Human papillomavirus type 16]] | | [[Category: Human papillomavirus type 16]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Mus musculus]] | | [[Category: Mus musculus]] |
| - | [[Category: Guan, J]] | + | [[Category: Guan J]] |
| - | [[Category: Hafenstein, S]] | + | [[Category: Hafenstein S]] |
| - | [[Category: L1 capsomer]]
| + | |
| - | [[Category: L1 pentamer]]
| + | |
| - | [[Category: Quasi-hpv16]]
| + | |
| - | [[Category: Rosie online]]
| + | |
| - | [[Category: Virus-immune system complex]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Cryo-electron microscopy (cryo-EM) was used to solve the structures of human papillomavirus type 16 (HPV16) complexed with fragments of antibody (Fab) from three different neutralizing monoclonals (mAbs): H16.1A, H16.14J, and H263.A2. The structure-function analysis revealed predominantly monovalent binding of each Fab with capsid interactions that involved multiple loops from symmetry related copies of the major capsid protein. The residues identified in each Fab-virus interface map to a conformational groove on the surface of the capsomer. In addition to the known involvement of the FG and HI loops, the DE loop was also found to constitute the core of each epitope. Surprisingly, the epitope mapping also identified minor contributions by EF and BC loops. Complementary immunological assays included mAb and Fab neutralization. The specific binding characteristics of mAbs correlated with different neutralizing behaviors in pre- and post-attachment neutralization assays.
Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization.,Guan J, Bywaters SM, Brendle SA, Lee H, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S Virology. 2015 May 18;483:253-263. doi: 10.1016/j.virol.2015.04.016. PMID:25996608[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Guan J, Bywaters SM, Brendle SA, Lee H, Ashley RE, Makhov AM, Conway JF, Christensen ND, Hafenstein S. Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization. Virology. 2015 May 18;483:253-263. doi: 10.1016/j.virol.2015.04.016. PMID:25996608 doi:http://dx.doi.org/10.1016/j.virol.2015.04.016
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