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| | ==Structure of ankyrin repeat protein== | | ==Structure of ankyrin repeat protein== |
| - | <StructureSection load='4o60' size='340' side='right' caption='[[4o60]], [[Resolution|resolution]] 2.52Å' scene=''> | + | <StructureSection load='4o60' size='340' side='right'caption='[[4o60]], [[Resolution|resolution]] 2.52Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4o60]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O60 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4o60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O60 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qfv|4qfv]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o60 OCA], [http://pdbe.org/4o60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o60 RCSB], [http://www.ebi.ac.uk/pdbsum/4o60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o60 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o60 OCA], [https://pdbe.org/4o60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o60 RCSB], [https://www.ebi.ac.uk/pdbsum/4o60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o60 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ethayathulla, A S]] | + | [[Category: Large Structures]] |
| - | [[Category: Guan, L]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Ankyrin]] | + | [[Category: Ethayathulla AS]] |
| - | [[Category: De novo protein]] | + | [[Category: Guan L]] |
| - | [[Category: Designed ankyrin repeat]]
| + | |
| Structural highlights
Publication Abstract from PubMed
A highly diverse DNA library coding for ankyrin seven-repeat proteins (ANK-N5C) was designed and constructed by a PCR-based combinatorial assembly strategy. A bacterial melibiose fermentation assay was adapted for in vivo functional screen. We isolated a transcription blocker that completely inhibits the melibiose-dependent expression of alpha-galactosidase (MelA) and melibiose permease (MelB) of Escherichia coli by specifically preventing activation of the melAB operon. High-resolution crystal structural determination reveals that the designed ANK-N5C protein has a typical ankyrin fold, and the specific transcription blocker, ANK-N5C-281, forms a domain-swapped dimer. Functional tests suggest that the activity of MelR, a DNA-binding transcription activator and a member of AraC family of transcription factors, is inhibited by ANK-N5C-281 protein. All ANK-N5C proteins are expected to have a concave binding area with negative surface potential, suggesting that the designed ANK-N5C library proteins may facilitate the discovery of binders recognizing structural motifs with positive surface potential, like in DNA-binding proteins. Overall, our results show that the established library is a useful tool for the discovery of novel bioactive reagents.
A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen.,Tikhonova EB, Ethayathulla AS, Su Y, Hariharan P, Xie S, Guan L Sci Rep. 2015 Jan 28;5:8070. doi: 10.1038/srep08070. PMID:25627011[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tikhonova EB, Ethayathulla AS, Su Y, Hariharan P, Xie S, Guan L. A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo functional screen. Sci Rep. 2015 Jan 28;5:8070. doi: 10.1038/srep08070. PMID:25627011 doi:http://dx.doi.org/10.1038/srep08070
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