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| | ==Hepatitis E Virus E2s domain (Genotype I) in complex with a neutralizing antibody 8G12== | | ==Hepatitis E Virus E2s domain (Genotype I) in complex with a neutralizing antibody 8G12== |
| - | <StructureSection load='4plk' size='340' side='right' caption='[[4plk]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <StructureSection load='4plk' size='340' side='right'caption='[[4plk]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4plk]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PLK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4plk]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Orthohepevirus_A Orthohepevirus A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLK FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4plj|4plj]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4plk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plk OCA], [http://pdbe.org/4plk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4plk RCSB], [http://www.ebi.ac.uk/pdbsum/4plk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4plk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4plk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4plk OCA], [https://pdbe.org/4plk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4plk RCSB], [https://www.ebi.ac.uk/pdbsum/4plk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4plk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L0L7P5_HEV L0L7P5_HEV] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Mus musculus]] | | [[Category: Mus musculus]] |
| - | [[Category: Li, S W]] | + | [[Category: Orthohepevirus A]] |
| - | [[Category: Sivaraman, J]] | + | [[Category: Li SW]] |
| - | [[Category: Tang, X H]] | + | [[Category: Sivaraman J]] |
| - | [[Category: Complex]] | + | [[Category: Tang XH]] |
| - | [[Category: Neutralizing antibody]]
| + | |
| - | [[Category: Viral protein-immune system complex]]
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| Structural highlights
Function
L0L7P5_HEV
Publication Abstract from PubMed
Hepatitis E virus (HEV), a non-enveloped, positive-sense, single-stranded RNA virus, is a major cause of enteric hepatitis. Classified into the family Hepeviridae, HEV comprises four genotypes (genotypes 1-4), which belong to a single serotype. We describe a monoclonal antibody (mAb), 8G12, which equally recognizes all four genotypes of HEV, with approximately 2.53-3.45 nM binding affinity. The mAb 8G12 has a protective, neutralizing capacity, which can significantly block virus infection in host cells. Animal studies with genotypes 1, 3 and 4 confirmed the cross-genotype neutralizing capacity of 8G12 and its effective prevention of hepatitis E disease. The complex crystal structures of 8G12 with the HEV E2s domain (the most protruded region of the virus capsid) of the abundant genotypes 1 and 4 were determined at 4.0 and 2.3 A resolution, respectively. These structures revealed that 8G12 recognizes both genotypes through the epitopes in the E2s dimerization region. Structure-based mutagenesis and cell-model assays with virus-like particles identified several conserved residues (Glu549, Lys554 and Gly591) that are essential for 8G12 neutralization. Moreover, the epitope of 8G12 is identified as a key epitope involved in virus-host interactions. These findings will help develop a common strategy for the prevention of the most abundant form of HEV infection.Cell Research advance online publication 20 March 2015; doi:10.1038/cr.2015.34.
Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody.,Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S, Xia N, Sivaraman J Cell Res. 2015 Mar 20. doi: 10.1038/cr.2015.34. PMID:25793314[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S, Xia N, Sivaraman J. Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 Mar 20. doi: 10.1038/cr.2015.34. PMID:25793314 doi:http://dx.doi.org/10.1038/cr.2015.34
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