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| | ==The mouse nucleosome structure containing H3.1== | | ==The mouse nucleosome structure containing H3.1== |
| - | <StructureSection load='5b1m' size='340' side='right' caption='[[5b1m]], [[Resolution|resolution]] 2.34Å' scene=''> | + | <StructureSection load='5b1m' size='340' side='right'caption='[[5b1m]], [[Resolution|resolution]] 2.34Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5b1m]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B1M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B1M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5b1m]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B1M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B1M FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5b1l|5b1l]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.34Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b1m OCA], [http://pdbe.org/5b1m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b1m RCSB], [http://www.ebi.ac.uk/pdbsum/5b1m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b1m ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b1m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b1m OCA], [https://pdbe.org/5b1m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b1m RCSB], [https://www.ebi.ac.uk/pdbsum/5b1m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b1m ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/H2B3A_MOUSE H2B3A_MOUSE]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. [[http://www.uniprot.org/uniprot/H2A1_MOUSE H2A1_MOUSE]] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | + | [https://www.uniprot.org/uniprot/H31_MOUSE H31_MOUSE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5b1m" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5b1m" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Histone 3D structures|Histone 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Horikoshi, N]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Kurumizaka, H]] | + | [[Category: Large Structures]] |
| - | [[Category: Machida, S]] | + | [[Category: Mus musculus]] |
| - | [[Category: Osakabe, A]] | + | [[Category: Horikoshi N]] |
| - | [[Category: Tachiwana, H]] | + | [[Category: Kurumizaka H]] |
| - | [[Category: Taguchi, H]] | + | [[Category: Machida S]] |
| - | [[Category: Urahama, T]] | + | [[Category: Osakabe A]] |
| - | [[Category: Chromatin]] | + | [[Category: Tachiwana H]] |
| - | [[Category: Dna binding]] | + | [[Category: Taguchi H]] |
| - | [[Category: Histone-fold]] | + | [[Category: Urahama T]] |
| - | [[Category: Structural protein-dna complex]]
| + | |
| Structural highlights
Function
H31_MOUSE
Publication Abstract from PubMed
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.
Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis.,Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, Yamagata K Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. PMID:28099840[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, Yamagata K. Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. PMID:28099840 doi:http://dx.doi.org/10.1016/j.celrep.2016.12.065
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