5i3c

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==Crystal structure of E.coli purine nucleoside phosphorylase with acycloguanosine==
==Crystal structure of E.coli purine nucleoside phosphorylase with acycloguanosine==
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<StructureSection load='5i3c' size='340' side='right' caption='[[5i3c]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
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<StructureSection load='5i3c' size='340' side='right'caption='[[5i3c]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5i3c]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I3C FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5i3c]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O139:H28_str._E24377A Escherichia coli O139:H28 str. E24377A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I3C FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC2:9-HYROXYETHOXYMETHYLGUANINE'>AC2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.32&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC2:9-HYROXYETHOXYMETHYLGUANINE'>AC2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i3c OCA], [http://pdbe.org/5i3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i3c RCSB], [http://www.ebi.ac.uk/pdbsum/5i3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i3c ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i3c OCA], [https://pdbe.org/5i3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i3c RCSB], [https://www.ebi.ac.uk/pdbsum/5i3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i3c ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DEOD_ECO24 DEOD_ECO24]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Escherichia coli purine nucleoside phosphorylase (PNP), which catalyzes the reversible phosphorolysis of purine ribonucleosides, belongs to the family I hexameric PNPs. Owing to their key role in the purine salvage pathway, PNPs are attractive targets for drug design against some pathogens. Acyclovir (ACV) is an acyclic derivative of the PNP substrate guanosine and is used as an antiviral drug for the treatment of some human viral infections. The crystalline complex of E. coli PNP with acyclovir was prepared by co-crystallization in microgravity using counter-diffusion through a gel layer in a capillary. The structure of the E. coli PNP-ACV complex was solved at 2.32 A resolution using the molecular-replacement method. The ACV molecule is observed in two conformations and sulfate ions were located in both the nucleoside-binding and phosphate-binding pockets of the enzyme. A comparison with the complexes of other hexameric and trimeric PNPs with ACV shows the similarity in acyclovir binding by these enzymes.
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Crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with acyclovir.,Timofeev VI, Zhukhlistova NE, Abramchik YA, Muravieva TI, Esipov RS, Kuranova IP Acta Crystallogr F Struct Biol Commun. 2018 Jul 1;74(Pt 7):402-409. doi:, 10.1107/S2053230X18008087. Epub 2018 Jun 26. PMID:29969103<ref>PMID:29969103</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5i3c" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Purine nucleoside phosphorylase 3D structures|Purine nucleoside phosphorylase 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Purine-nucleoside phosphorylase]]
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[[Category: Escherichia coli O139:H28 str. E24377A]]
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[[Category: Abramchik, Y A]]
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[[Category: Large Structures]]
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[[Category: Esipov, R S]]
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[[Category: Abramchik YA]]
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[[Category: Kuranova, I P]]
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[[Category: Esipov RS]]
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[[Category: Timofeev, V I]]
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[[Category: Kuranova IP]]
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[[Category: Complex]]
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[[Category: Timofeev VI]]
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[[Category: Transferase]]
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Current revision

Crystal structure of E.coli purine nucleoside phosphorylase with acycloguanosine

PDB ID 5i3c

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