1td0

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[[Image:1td0.jpg|left|200px]]
 
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{{Structure
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==Viral capsid protein SHP at pH 5.5==
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|PDB= 1td0 |SIZE=350|CAPTION= <scene name='initialview01'>1td0</scene>, resolution 1.95&Aring;
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<StructureSection load='1td0' size='340' side='right'caption='[[1td0]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1td0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_P21 Enterobacteria phage P21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TD0 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1td0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1td0 OCA], [https://pdbe.org/1td0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1td0 RCSB], [https://www.ebi.ac.uk/pdbsum/1td0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1td0 ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1td0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1td0 OCA], [http://www.ebi.ac.uk/pdbsum/1td0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1td0 RCSB]</span>
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[https://www.uniprot.org/uniprot/DECO_BPP21 DECO_BPP21] Stabilizes the head shell following the rearrangement of the gp7 subunits of the head shell lattice that accompanies expansion of the head.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''Viral capsid protein SHP at pH 5.5'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/td/1td0_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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SHP, the capsid-stabilizing protein of lambdoid phage 21, is highly resistant against denaturant-induced unfolding. We demonstrate that this high functional stability of SHP is due to a high kinetic stability with a half-life for unfolding of 25 days at zero denaturant, while the thermodynamic stability is not unusually high. Unfolding experiments demonstrated that the trimeric state (also observed in crystals and present on the phage capsid) of SHP is kinetically stable in solution, while the monomer intermediate unfolds very rapidly. We also determined the crystal structure of trimeric SHP at 1.5A resolution, which was compared to that of its functional homolog gpD. This explains how a tight network of H-bonds rigidifies crucial interpenetrating residues, leading to the observed extremely slow trimer dissociation or denaturation. Taken as a whole, our results provide molecular-level insights into natural strategies to achieve kinetic stability by taking advantage of protein oligomerization. Kinetic stability may be especially needed in phage capsids to allow survival in harsh environments.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1td0 ConSurf].
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1TD0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TD0 OCA].
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<div style="clear:both"></div>
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__TOC__
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==Reference==
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</StructureSection>
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Kinetic stability and crystal structure of the viral capsid protein SHP., Forrer P, Chang C, Ott D, Wlodawer A, Pluckthun A, J Mol Biol. 2004 Nov 12;344(1):179-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15504410 15504410]
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[[Category: Enterobacteria phage P21]]
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[[Category: Enterobacteria phage p21]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Chang C]]
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[[Category: Chang, C.]]
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[[Category: Forrer P]]
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[[Category: Forrer, P.]]
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[[Category: Ott D]]
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[[Category: Ott, D.]]
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[[Category: Plueckthun A]]
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[[Category: Plueckthun, A.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: shp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:54:25 2008''
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Current revision

Viral capsid protein SHP at pH 5.5

PDB ID 1td0

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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