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| | ==Crystal structure of the monomeric, cleaved form of the Pore-Forming Toxin Monalysin== | | ==Crystal structure of the monomeric, cleaved form of the Pore-Forming Toxin Monalysin== |
| - | <StructureSection load='4mko' size='340' side='right' caption='[[4mko]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4mko' size='340' side='right'caption='[[4mko]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4mko]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MKO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MKO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4mko]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_entomophila_L48 Pseudomonas entomophila L48]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MKO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mjt|4mjt]], [[4mkq|4mkq]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mko OCA], [http://pdbe.org/4mko PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mko RCSB], [http://www.ebi.ac.uk/pdbsum/4mko PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mko ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mko OCA], [https://pdbe.org/4mko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mko RCSB], [https://www.ebi.ac.uk/pdbsum/4mko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mko ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/MONAL_PSEE4 MONAL_PSEE4] Pore-forming toxin that contributes to the virulence of P.entomophila against Drosophila, playing an important role in host intestinal damage and lethality. Displays cytolytic and hemolytic activity.<ref>PMID:21980286</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Leone, P]] | + | [[Category: Large Structures]] |
| - | [[Category: Roussel, A]] | + | [[Category: Pseudomonas entomophila L48]] |
| - | [[Category: Pore-forming toxin]] | + | [[Category: Leone P]] |
| - | [[Category: Toxin]] | + | [[Category: Roussel A]] |
| Structural highlights
Function
MONAL_PSEE4 Pore-forming toxin that contributes to the virulence of P.entomophila against Drosophila, playing an important role in host intestinal damage and lethality. Displays cytolytic and hemolytic activity.[1]
Publication Abstract from PubMed
beta-barrel pore-forming toxins (beta-PFT), a large family of bacterial toxins, are generally secreted as water-soluble monomers and can form oligomeric pores in membranes following proteolytic cleavage and interaction with cell surface receptors. Monalysin has been recently identified as a beta-PFT that contributes to the virulence of Pseudomonas entomophila against Drosophila. It is secreted as a pro-protein that becomes active upon cleavage. Here we report the crystal and cryo electron microscopy structure of the pro-form of Monalysin as well as the crystal structures of the cleaved form and of an inactive mutant lacking the membrane-spanning region. The overall structure of Monalysin displays an elongated shape, which resembles those of beta-pore-forming toxins, such as aerolysin, but is devoid of receptor binding domain. X-ray crystallography, cryo electron microscopy and light-scattering studies show that pro-Monalysin forms a stable doughnut-like 18-mer complex composed of two disk-shaped nonamers held together by N-terminal swapping of the pro-peptides. This observation is in contrast with the monomeric pro-form of the other beta-PFTs that are receptor dependent for membrane interaction. The membrane-spanning region of pro-monalysin is fully buried in the center of the doughnut suggesting that upon pro-peptides cleavage, the two disk-shaped nonamers can - and have to - dissociate to leave the transmembrane segments free to deploy and lead to pore formation. In contrast with other toxins, the delivery of 18 subunits at once, nearby the cell surface, may be used to by-pass the requirement of receptor-dependent concentration to reach the threshold for oligomerization into the pore forming complex.
X-ray and Cryo-electron Microscopy Structures of Monalysin Pore Forming Toxin Reveals Multimerization of the Pro-Form.,Leone P, Bebeacua C, Opota O, Kellenberger C, Klaholz B, Orlov I, Cambillau C, Lemaitre B, Roussel A J Biol Chem. 2015 Apr 5. pii: jbc.M115.646109. PMID:25847242[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Opota O, Vallet-Gely I, Vincentelli R, Kellenberger C, Iacovache I, Gonzalez MR, Roussel A, van der Goot FG, Lemaitre B. Monalysin, a novel ss-pore-forming toxin from the Drosophila pathogen Pseudomonas entomophila, contributes to host intestinal damage and lethality. PLoS Pathog. 2011 Sep;7(9):e1002259. doi: 10.1371/journal.ppat.1002259. Epub 2011 , Sep 29. PMID:21980286 doi:http://dx.doi.org/10.1371/journal.ppat.1002259
- ↑ Leone P, Bebeacua C, Opota O, Kellenberger C, Klaholz B, Orlov I, Cambillau C, Lemaitre B, Roussel A. X-ray and Cryo-electron Microscopy Structures of Monalysin Pore Forming Toxin Reveals Multimerization of the Pro-Form. J Biol Chem. 2015 Apr 5. pii: jbc.M115.646109. PMID:25847242 doi:http://dx.doi.org/10.1074/jbc.M115.646109
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