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5wtz

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Crystal structure of C. perfringens iota-like enterotoxin CPILE-a with NAD+

Structural highlights

5wtz is a 1 chain structure with sequence from Clostridium perfringens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.803Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

X5I2D7_CLOPF

Publication Abstract from PubMed

Unusual outbreaks of food poisoning in Japan were reported in which Clostridium perfringens was strongly suspected to be the cause based on epidemiological information and fingerprinting of isolates. The isolated strains lack the typical C. perfringens enterotoxin (CPE) but secrete a new enterotoxin consisting of two components: C. perfringens iota-like enterotoxin-a (CPILE-a), which acts as an enzymatic ADP-ribosyltransferase, and CPILE-b, a membrane binding component. Here we present the crystal structures of apo-CPILE-a, NAD+-CPILE-a and NADH-CPILE-a. Though CPILE-a structure has high similarity with known iota toxin-a (Ia) with NAD+, it possesses two extra-long protruding loops from G262-S269 and E402-K408 that are distinct from Ia. Based on the Ia-actin complex structure, we focused on actin-binding interface regions (I-V) including two protruding loops (PT) and examined how mutations in these regions affect the ADP-ribosylation activity of CPILE-a. Though some site-directed mutagenesis studies have already been conducted on the actin binding site of Ia, in the present study, mutagenesis studies were conducted against both alpha- and beta/gamma-actin in CPILE-a and Ia. Interestingly, CPILE-a ADP-ribosylates both alpha- and beta/gamma-actin, but its sensitivity towards beta/gamma-actin is 36% compared with alpha-actin. Our results contrast to that only C2-I ADP-ribosylates beta/gamma-actin. We also showed that PT-I and two convex-concave interactions in CPILE-a are important for actin binding. The current study is the first detailed analysis of site-directed mutagenesis in the actin binding region of Ia and CPILE-a against both alpha- and beta/gamma-actin.

Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin.,Toniti W, Yoshida T, Tsurumura T, Irikura D, Monma C, Kamata Y, Tsuge H PLoS One. 2017 Feb 15;12(2):e0171278. doi: 10.1371/journal.pone.0171278., eCollection 2017. PMID:28199340^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Toniti W, Yoshida T, Tsurumura T, Irikura D, Monma C, Kamata Y, Tsuge H. Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin. PLoS One. 2017 Feb 15;12(2):e0171278. doi: 10.1371/journal.pone.0171278., eCollection 2017. PMID:28199340 doi:http://dx.doi.org/10.1371/journal.pone.0171278

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wtz"

@@ Line 1: / Line 1: @@
 ==Crystal structure of C. perfringens iota-like enterotoxin CPILE-a with NAD+==
-<StructureSection load='5wtz' size='340' side='right' caption='[[5wtz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='5wtz' size='340' side='right'caption='[[5wtz]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wtz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WTZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wtz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.803&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wu0|5wu0]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtz OCA], [http://pdbe.org/5wtz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtz RCSB], [http://www.ebi.ac.uk/pdbsum/5wtz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtz OCA], [https://pdbe.org/5wtz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtz RCSB], [https://www.ebi.ac.uk/pdbsum/5wtz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtz ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/X5I2D7_CLOPF X5I2D7_CLOPF]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Irikura, D]]
+[[Category: Clostridium perfringens]]
-[[Category: Toniti, W]]
+[[Category: Large Structures]]
-[[Category: Tsuge, H]]
+[[Category: Irikura D]]
-[[Category: Tsurumura, T]]
+[[Category: Toniti W]]
-[[Category: Yoshida, T]]
+[[Category: Tsuge H]]
-[[Category: Actin]]
+[[Category: Tsurumura T]]
-[[Category: Adp-ribosylation]]
+[[Category: Yoshida T]]
-[[Category: Bacterial toxin]]
-[[Category: Cpile-a]]
-[[Category: Toxin]]

5wtz

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Current revision

Crystal structure of C. perfringens iota-like enterotoxin CPILE-a with NAD+

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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