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5ncc
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5ncc is ON HOLD Authors: ARNOUX, P., SORIGUE, D., BEISSON, F., PIGNOL, D. Description: Structure of Alkane Photosynthase in complex with FAD and pa...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid== | |
| + | <StructureSection load='5ncc' size='340' side='right'caption='[[5ncc]], [[Resolution|resolution]] 3.12Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ncc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlorella_variabilis Chlorella variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NCC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.12Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ncc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ncc OCA], [https://pdbe.org/5ncc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ncc RCSB], [https://www.ebi.ac.uk/pdbsum/5ncc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ncc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FAP_CHLVA FAP_CHLVA] Catalyzes the decarboxylation of free fatty acids to n-alkanes or n-alkenes in response to blue light (PubMed:28860382, PubMed:30106504, PubMed:30673222). Substrate preference is toward fatty acids with C16 or C17 chains (PubMed:28860382, PubMed:30106504, PubMed:30673222). Saturated fatty acids are converted to alkanes, not alkenes (PubMed:28860382). The decarboxylation is initiated through electron abstraction from the fatty acid by the photo-excited FAD (PubMed:28860382).<ref>PMID:28860382</ref> <ref>PMID:30106504</ref> <ref>PMID:30673222</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although many organisms capture or respond to sunlight, few enzymes are known to be driven by light. Among these are DNA photolyases and the photosynthetic reaction centers. Here, we show that the microalga Chlorella variabilis NC64A harbors a photoenzyme that acts in lipid metabolism. This enzyme belongs to an algae-specific clade of the glucose-methanol-choline oxidoreductase family and catalyzes the decarboxylation of free fatty acids to n-alkanes or -alkenes in response to blue light. Crystal structure of the protein reveals a fatty acid-binding site in a hydrophobic tunnel leading to the light-capturing flavin adenine dinucleotide (FAD) cofactor. The decarboxylation is initiated through electron abstraction from the fatty acid by the photoexcited FAD with a quantum yield >80%. This photoenzyme, which we name fatty acid photodecarboxylase, may be useful in light-driven, bio-based production of hydrocarbons. | ||
| - | + | An algal photoenzyme converts fatty acids to hydrocarbons.,Sorigue D, Legeret B, Cuine S, Blangy S, Moulin S, Billon E, Richaud P, Brugiere S, Coute Y, Nurizzo D, Muller P, Brettel K, Pignol D, Arnoux P, Li-Beisson Y, Peltier G, Beisson F Science. 2017 Sep 1;357(6354):903-907. doi: 10.1126/science.aan6349. PMID:28860382<ref>PMID:28860382</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Arnoux | + | <div class="pdbe-citations 5ncc" style="background-color:#fffaf0;"></div> |
| - | [[Category: Beisson | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Chlorella variabilis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arnoux P]] | ||
| + | [[Category: Beisson F]] | ||
| + | [[Category: Pignol D]] | ||
| + | [[Category: Sorigue D]] | ||
Current revision
Structure of Fatty acid Photodecarboxylase in complex with FAD and palmitic acid
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