1tjy

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[[Image:1tjy.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF==
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|PDB= 1tjy |SIZE=350|CAPTION= <scene name='initialview01'>1tjy</scene>, resolution 1.30&Aring;
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<StructureSection load='1tjy' size='340' side='right'caption='[[1tjy]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PAV:(2R,4S)-2-METHYL-2,3,3,4-TETRAHYDROXYTETRAHYDROFURAN'>PAV</scene>
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<table><tr><td colspan='2'>[[1tjy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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|GENE= LsrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAV:(2R,4S)-2-METHYL-2,3,3,4-TETRAHYDROXYTETRAHYDROFURAN'>PAV</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjy OCA], [https://pdbe.org/1tjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tjy RCSB], [https://www.ebi.ac.uk/pdbsum/1tjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tjy ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1jx6|1JX6]], [[1tm2|1TM2]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tjy OCA], [http://www.ebi.ac.uk/pdbsum/1tjy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tjy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/LSRB_SALTY LSRB_SALTY] Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and LsrD permeases (Probable).<ref>PMID:11722742</ref>
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== Evolutionary Conservation ==
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'''Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tjy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tjy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Bacterial populations use cell-cell communication to coordinate community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is mediated by small molecule signals known as autoinducers. While most autoinducers are species specific, autoinducer-2 (AI-2), first identified in the marine bacterium Vibrio harveyi, is produced and detected by many Gram-negative and Gram-positive bacteria. The crystal structure of the V. harveyi AI-2 signaling molecule bound to its receptor protein revealed an unusual furanosyl borate diester. Here, we present the crystal structure of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium. We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks boron. Our results demonstrate that two different species of bacteria recognize two different forms of the autoinducer signal, both derived from 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the chemical lexicon used by bacteria in interspecies signaling.
Bacterial populations use cell-cell communication to coordinate community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is mediated by small molecule signals known as autoinducers. While most autoinducers are species specific, autoinducer-2 (AI-2), first identified in the marine bacterium Vibrio harveyi, is produced and detected by many Gram-negative and Gram-positive bacteria. The crystal structure of the V. harveyi AI-2 signaling molecule bound to its receptor protein revealed an unusual furanosyl borate diester. Here, we present the crystal structure of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium. We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks boron. Our results demonstrate that two different species of bacteria recognize two different forms of the autoinducer signal, both derived from 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the chemical lexicon used by bacteria in interspecies signaling.
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==About this Structure==
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Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2.,Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, Bassler BL, Hughson FM Mol Cell. 2004 Sep 10;15(5):677-87. PMID:15350213<ref>PMID:15350213</ref>
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1TJY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJY OCA].
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==Reference==
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Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2., Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, Bassler BL, Hughson FM, Mol Cell. 2004 Sep 10;15(5):677-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15350213 15350213]
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[[Category: Salmonella typhimurium lt2]]
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[[Category: Single protein]]
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[[Category: Bassler, B L.]]
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[[Category: Campagna, S R.]]
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[[Category: Hughson, F M.]]
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[[Category: Miller, S T.]]
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[[Category: Semmelhack, M F.]]
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[[Category: Taga, M E.]]
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[[Category: Xavier, K B.]]
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[[Category: protein-ligand complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:57:10 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1tjy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Bassler BL]]
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[[Category: Campagna SR]]
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[[Category: Hughson FM]]
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[[Category: Miller ST]]
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[[Category: Semmelhack MF]]
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[[Category: Taga ME]]
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[[Category: Xavier KB]]

Current revision

Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF

PDB ID 1tjy

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