1tkv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:12, 22 May 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1tkv.jpg|left|200px]]
 
-
{{Structure
+
==Solution Structure of T4 AsiA Dimer==
-
|PDB= 1tkv |SIZE=350|CAPTION= <scene name='initialview01'>1tkv</scene>
+
<StructureSection load='1tkv' size='340' side='right'caption='[[1tkv]]' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1tkv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1ka3 1ka3]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TKV FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
|GENE= ASIA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkv OCA], [https://pdbe.org/1tkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tkv RCSB], [https://www.ebi.ac.uk/pdbsum/1tkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tkv ProSAT]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tkv OCA], [http://www.ebi.ac.uk/pdbsum/1tkv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tkv RCSB]</span>
+
[https://www.uniprot.org/uniprot/ASIA_BPT4 ASIA_BPT4] Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.<ref>PMID:8021178</ref> <ref>PMID:15257291</ref>
-
}}
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
'''Solution Structure of T4 AsiA Dimer'''
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tkv_consurf.spt"</scriptWhenChecked>
-
==Overview==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tkv ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
The bacteriophage T4 AsiA protein is a multifunctional protein that simultaneously acts as both a repressor and activator of gene expression during the phage life cycle. These dual roles with opposing transcriptional consequences are achieved by modification of the host RNA polymerase in which AsiA binds to conserved region 4 (SR4) of sigma(70), altering the pathway of promoter selection by the holoenzyme. The mechanism by which AsiA flips this genetic switch has now been revealed, in part, from the three-dimensional structure of AsiA and the elucidation of its interaction with SR4. The structure of AsiA is that of a novel homodimer in which each monomer is constructed as a seven-helix bundle arranged in four overlapping helix-loop-helix elements. Identification of the protein interfaces for both the AsiA homodimer and the AsiA-sigma(70) complex reveals that these interfaces are coincident. Thus, the AsiA interaction with sigma(70) necessitates that the AsiA homodimer dissociate to form an AsiA-SR4 heterodimer, exchanging one protein subunit for another to alter promoter choice by RNA polymerase.
The bacteriophage T4 AsiA protein is a multifunctional protein that simultaneously acts as both a repressor and activator of gene expression during the phage life cycle. These dual roles with opposing transcriptional consequences are achieved by modification of the host RNA polymerase in which AsiA binds to conserved region 4 (SR4) of sigma(70), altering the pathway of promoter selection by the holoenzyme. The mechanism by which AsiA flips this genetic switch has now been revealed, in part, from the three-dimensional structure of AsiA and the elucidation of its interaction with SR4. The structure of AsiA is that of a novel homodimer in which each monomer is constructed as a seven-helix bundle arranged in four overlapping helix-loop-helix elements. Identification of the protein interfaces for both the AsiA homodimer and the AsiA-sigma(70) complex reveals that these interfaces are coincident. Thus, the AsiA interaction with sigma(70) necessitates that the AsiA homodimer dissociate to form an AsiA-SR4 heterodimer, exchanging one protein subunit for another to alter promoter choice by RNA polymerase.
-
==About this Structure==
+
Flipping a genetic switch by subunit exchange.,Lambert LJ, Schirf V, Demeler B, Cadene M, Werner MH EMBO J. 2001 Dec 17;20(24):7149-59. PMID:11742991<ref>PMID:11742991</ref>
-
1TKV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. This structure supersedes the now removed PDB entry 1KA3. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TKV OCA].
+
-
 
+
-
==Reference==
+
-
Flipping a genetic switch by subunit exchange., Lambert LJ, Schirf V, Demeler B, Cadene M, Werner MH, EMBO J. 2001 Dec 17;20(24):7149-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11742991 11742991]
+
-
[[Category: Enterobacteria phage t4]]
+
-
[[Category: Single protein]]
+
-
[[Category: Cadene, M.]]
+
-
[[Category: Demeler, B.]]
+
-
[[Category: Lambert, L J.]]
+
-
[[Category: Schirf, V.]]
+
-
[[Category: Werner, M H.]]
+
-
[[Category: anti-sigma]]
+
-
[[Category: homodimer]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:57:29 2008''
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1tkv" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Escherichia virus T4]]
 +
[[Category: Large Structures]]
 +
[[Category: Cadene M]]
 +
[[Category: Demeler B]]
 +
[[Category: Lambert LJ]]
 +
[[Category: Schirf V]]
 +
[[Category: Werner MH]]

Current revision

Solution Structure of T4 AsiA Dimer

PDB ID 1tkv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tkv"
Personal tools