1tlh

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[[Image:1tlh.jpg|left|200px]]
 
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{{Structure
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==T4 AsiA bound to sigma70 region 4==
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|PDB= 1tlh |SIZE=350|CAPTION= <scene name='initialview01'>1tlh</scene>
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<StructureSection load='1tlh' size='340' side='right'caption='[[1tlh]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1tlh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TLH FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE= ASIA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4]), RPOD, ALT, B3067 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlh OCA], [https://pdbe.org/1tlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tlh RCSB], [https://www.ebi.ac.uk/pdbsum/1tlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tlh ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1tkv|1TKV]], [[1tl6|1TL6]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tlh OCA], [http://www.ebi.ac.uk/pdbsum/1tlh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tlh RCSB]</span>
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[https://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tlh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tlh ConSurf].
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<div style="clear:both"></div>
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'''T4 AsiA bound to sigma70 region 4'''
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==See Also==
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Bacteriophage T4 AsiA is a versatile transcription factor capable of inhibiting host gene expression as an 'anti-sigma' factor while simultaneously promoting gene-specific expression of T4 middle genes in conjunction with T4 MotA. To accomplish this task, AsiA engages conserved region 4 of Eschericia coli sigma70, blocking recognition of most host promoters by sequestering the DNA-binding surface at the AsiA/sigma70 interface. The three-dimensional structure of an AsiA/region 4 complex reveals that the C-terminal alpha helix of region 4 is unstructured, while four other helices adopt a completely different conformation relative to the canonical structure of unbound region 4. That AsiA induces, rather than merely stabilizes, this rearrangement can be realized by comparison to the homologous structures of region 4 solved in a variety of contexts, including the structure of Thermotoga maritima sigmaA region 4 described herein. AsiA simultaneously occupies the surface of region 4 that ordinarily contacts core RNA polymerase (RNAP), suggesting that an AsiA-bound sigma70 may also undergo conformational changes in the context of the RNAP holoenzyme.
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==About this Structure==
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1TLH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLH OCA].
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==Reference==
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T4 AsiA blocks DNA recognition by remodeling sigma70 region 4., Lambert LJ, Wei Y, Schirf V, Demeler B, Werner MH, EMBO J. 2004 Aug 4;23(15):2952-62. Epub 2004 Jul 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15257291 15257291]
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[[Category: Enterobacteria phage t4]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Escherichia virus T4]]
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[[Category: Demeler, B.]]
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[[Category: Large Structures]]
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[[Category: Lambert, L J.]]
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[[Category: Demeler B]]
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[[Category: Schirf, V.]]
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[[Category: Lambert LJ]]
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[[Category: Wei, Y.]]
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[[Category: Schirf V]]
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[[Category: Werner, M H.]]
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[[Category: Wei Y]]
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[[Category: anti-sigma]]
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[[Category: Werner MH]]
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[[Category: nmr]]
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[[Category: rna polymerase]]
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[[Category: sigma70]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:57:44 2008''
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Current revision

T4 AsiA bound to sigma70 region 4

PDB ID 1tlh

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