5v00
From Proteopedia
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==Structure of HutD from Pseudomonas fluorescens SBW25 (Formate condition)== | ==Structure of HutD from Pseudomonas fluorescens SBW25 (Formate condition)== | ||
| - | <StructureSection load='5v00' size='340' side='right' caption='[[5v00]], [[Resolution|resolution]] 1.80Å' scene=''> | + | <StructureSection load='5v00' size='340' side='right'caption='[[5v00]], [[Resolution|resolution]] 1.80Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5v00]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3esg 3esg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V00 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[5v00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens_SBW25 Pseudomonas fluorescens SBW25]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3esg 3esg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V00 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v00 OCA], [https://pdbe.org/5v00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v00 RCSB], [https://www.ebi.ac.uk/pdbsum/5v00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v00 ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C3K802_PSEFS C3K802_PSEFS] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cupins form one of the most functionally diverse superfamilies of proteins, with members performing a wide range of catalytic, non-catalytic, and regulatory functions. HutD is a predicted bicupin protein that is involved in histidine utilization (Hut) in Pseudomonas species. Previous genetic analyses have suggested that it limits the upper level of Hut pathway expression, but its mechanism of action is unknown. Here, we have determined the structure of PfluHutD at 1.74 A resolution in several crystallization conditions, and identified N-formyl-L-glutamate (a Hut pathway intermediate) is a potential ligand in vivo. This article is protected by copyright. All rights reserved. | ||
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| + | Crystal structure of a bicupin protein HutD involved in histidine utilization in Pseudomonas.,Gerth ML, Liu Y, Jiao W, Zhang XX, Baker EN, Lott JS, Rainey PB, Johnston JM Proteins. 2017 Apr 6. doi: 10.1002/prot.25303. PMID:28383128<ref>PMID:28383128</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5v00" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Pseudomonas fluorescens SBW25]] |
| - | [[Category: | + | [[Category: Baker EN]] |
| - | [[Category: | + | [[Category: Gerth ML]] |
| - | [[Category: | + | [[Category: Johnston JM]] |
| - | [[Category: | + | [[Category: Liu Y]] |
| - | [[Category: | + | [[Category: Lott JS]] |
| - | [[Category: | + | [[Category: Rainey PB]] |
| - | + | ||
Current revision
Structure of HutD from Pseudomonas fluorescens SBW25 (Formate condition)
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