2oar

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{{Seed}}
 
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[[Image:2oar.png|left|200px]]
 
==Mechanosensitive Channel of Large Conductance (MscL)==
==Mechanosensitive Channel of Large Conductance (MscL)==
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<StructureSection load='2oar' size='340' side='right'caption='[[2oar]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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===Model of Channel Opening===
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== Structural highlights ==
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<applet load='1kyk' size='300' frame='true' align='right' caption='Theoretical Models'
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<table><tr><td colspan='2'>[[2oar]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. The November 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Mechanosensitive Channels'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_11 10.2210/rcsb_pdb/mom_2008_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OAR FirstGlance]. <br>
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scene='Goodsell_Sandbox/1kyl/1' />
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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Theoretical models<ref>The theoretical models were deposited in the [[PDB]] (before it stopped accepting theoretical models) as [[1kyk]], [[1kyl]], and [[1kym]].</ref> of the <scene name='Goodsell_Sandbox/1kyk/1'>closed</scene> (initial scene), <scene name='Goodsell_Sandbox/1kyl/1'>intermediate</scene>, and <scene name='Goodsell_Sandbox/1kym/1'>open</scene> forms of the channel have been generated based on data from electron paramagnetic resonance spectroscopy, using mutant forms of the protein with spin labels attached to engineered cysteine amino acids in the transmembrane segments<ref>Open channel structure of MscL and the gating mechanism of mechanosensitive channels. Perozo, E, Cortes DM, Sompornpisut, P, Kloda, A, Martinac, B. Nature 2002, 418, 942-948. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198539 12198539]</ref>.
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oar OCA], [https://pdbe.org/2oar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oar RCSB], [https://www.ebi.ac.uk/pdbsum/2oar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oar ProSAT]</span></td></tr>
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{{Clear}}
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</table>
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----
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== Function ==
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<br>
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[https://www.uniprot.org/uniprot/MSCL_MYCTA MSCL_MYCTA] Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell.[UniProtKB:P9WJN5]
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<!--
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== Evolutionary Conservation ==
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The FIRST line below this paragraph, containing "STRUCTURE_2oar", creates the "Structure Box" on the page.
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[[Image:Consurf_key_small.gif|200px|right]]
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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Check<jmol>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<jmolCheckbox>
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or leave the SCENE parameter empty for the default display.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oar_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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The SECOND line below this paragraph, {{ABSTRACT_PUBMED_9856938}}, adds the Publication Abstract to the page
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<text>to colour the structure by Evolutionary Conservation</text>
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(as it appears on PubMed at http://www.pubmed.gov), where 9856938 is the PubMed ID number.
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</jmolCheckbox>
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-->
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oar ConSurf].
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{{STRUCTURE_2oar| PDB=2oar | SCENE= }}
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<div style="clear:both"></div>
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{{ABSTRACT_PUBMED_9856938}}
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==About this Structure==
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2OAR <ref>Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel., Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC, Science. 1998 Dec 18;282(5397):2220-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9856938 9856938]</ref> is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1msl 1msl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAR OCA].
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==See Also==
==See Also==
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Many structural features of a related channel, MscS, including animated morphs of opening an closing, are illustrated at [[Mechanosensitive channels: opening and closing]].
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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__TOC__
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==References and Notes==
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</StructureSection>
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<references/>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Mechanosensitive Channels]]
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[[Category: Single protein]]
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[[Category: Mycobacterium tuberculosis H37Ra]]
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[[Category: Chang, G.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Lee, A T.]]
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[[Category: Chang G]]
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[[Category: Rees, D C.]]
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[[Category: Lee AT]]
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[[Category: Spencer, R H.]]
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[[Category: Rees DC]]
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[[Category: Steinbacher, S.]]
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[[Category: Spencer RH]]
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[[Category: Strop, P.]]
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[[Category: Steinbacher S]]
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[[Category: Stretch activated ion channel mechanosensitive]]
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[[Category: Strop P]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 20 12:12:19 2008''
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Current revision

Mechanosensitive Channel of Large Conductance (MscL)

PDB ID 2oar

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Proteopedia Page Contributors and Editors (what is this?)

OCA, Eric Martz, David S. Goodsell, Eran Hodis

Retrieved from "http://52.214.119.220/wiki/index.php/2oar"
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