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| | ==Crystal structure of the Kunitz-type protease inhibitor ShPI-1 Lys13Leu mutant in complex with pancreatic elastase== | | ==Crystal structure of the Kunitz-type protease inhibitor ShPI-1 Lys13Leu mutant in complex with pancreatic elastase== |
| - | <StructureSection load='3uou' size='340' side='right' caption='[[3uou]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='3uou' size='340' side='right'caption='[[3uou]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3uou]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Carribean_sea_anemone Carribean sea anemone] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UOU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3uou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stichodactyla_helianthus Stichodactyla helianthus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3UOU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uou OCA], [http://pdbe.org/3uou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3uou RCSB], [http://www.ebi.ac.uk/pdbsum/3uou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3uou ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3uou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uou OCA], [https://pdbe.org/3uou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3uou RCSB], [https://www.ebi.ac.uk/pdbsum/3uou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3uou ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG]] Acts upon elastin. [[http://www.uniprot.org/uniprot/ISH1_STOHE ISH1_STOHE]] Active against serine, cysteine, and aspartic proteinases. Can bind vertebrate trypsin and chymotrypsin.<ref>PMID:9027993</ref> <ref>PMID:22975140</ref> | + | [https://www.uniprot.org/uniprot/CELA1_PIG CELA1_PIG] Acts upon elastin. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 3uou" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 3uou" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Elastase 3D structures|Elastase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Carribean sea anemone]] | + | [[Category: Large Structures]] |
| - | [[Category: Pancreatic elastase]] | + | [[Category: Stichodactyla helianthus]] |
| | [[Category: Sus scrofa]] | | [[Category: Sus scrofa]] |
| - | [[Category: Betzel, C]] | + | [[Category: Betzel C]] |
| - | [[Category: Chavez, M A]] | + | [[Category: Chavez MA]] |
| - | [[Category: Garcia-Fernandez, R]] | + | [[Category: Garcia-Fernandez R]] |
| - | [[Category: Gonzalez-Gonzalez, Y]] | + | [[Category: Gonzalez-Gonzalez Y]] |
| - | [[Category: Perbandt, M]] | + | [[Category: Perbandt M]] |
| - | [[Category: Redecke, L]] | + | [[Category: Redecke L]] |
| - | [[Category: Rehders, D]] | + | [[Category: Rehders D]] |
| - | [[Category: Hydrolase-hydrolase inhibitor complex]]
| + | |
| - | [[Category: Kunitz-type inhibitor]]
| + | |
| - | [[Category: Protein-protein interaction]]
| + | |
| Structural highlights
Function
CELA1_PIG Acts upon elastin.
Publication Abstract from PubMed
Elastase-like enzymes are involved in important diseases, such as acute pancreatitis, chronic inflammatory lung diseases, and cancer. Structural insights into the interaction with specific inhibitors will contribute to develop novel anti-elastase compounds that resist rapid oxidation and proteolysis. Proteinaceous Kunitz-type inhibitors homologous to the bovine pancreatic trypsin inhibitor (BPTI) provide a suitable scaffold, but structural aspects of their interaction with elastase-like enzymes have not been elucidated. Here, we increased the selectivity of ShPI-1, a versatile serine protease inhibitor from the sea anemone Stichodactyla helianthus with high biomedical and biotechnological potential, towards elastase-like enzymes by substitution of the P1 residue (Lys13) with leucine. The variant (rShPI-1/K13L) exhibits a novel anti-porcine pancreatic elastase (PPE) activity, together with a significantly improved inhibition of human neuthrophil elastase (HNE) and chymotrypsin. The crystal structure of the PPE-rShPI-1/K13L complex determined at 2.0 A resolution provided first details of the canonical interaction between a BPTI-Kunitz-type domain and elastase-like enzymes. In addition to the essential impact of the variant P1 residue for complex stability, the interface is improved by increased contributions of the primary and secondary binding loop as compared to similar trypsin and chymotrypsin complexes. Comparison of the interaction network with elastase complexes of canonical inhibitors from the chelonianin family supports a key role of the P3 site in ShPI-1 in directing its selectivity against pancreatic and neutrophil elastases. Our results provide the structural basis for site-specific mutagenesis to further improve the binding affinity and/or to direct the selectivity of BPTI-Kunitz-type inhibitors towards elastase-like enzymes.
Three-dimensional structure of a Kunitz-type inhibitor in complex with an elastase-like enzyme.,Garcia-Fernandez R, Perbandt M, Rehders D, Ziegelmueller P, Piganeau N, Hahn U, Betzel C, de Los Angeles Chavez M, Redecke L J Biol Chem. 2015 Apr 15. pii: jbc.M115.647586. PMID:25878249[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Garcia-Fernandez R, Perbandt M, Rehders D, Ziegelmueller P, Piganeau N, Hahn U, Betzel C, de Los Angeles Chavez M, Redecke L. Three-dimensional structure of a Kunitz-type inhibitor in complex with an elastase-like enzyme. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647586. PMID:25878249 doi:http://dx.doi.org/10.1074/jbc.M115.647586
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