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4rl9

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==Crystal structure of Acinetobacter baumannii CarO1==
==Crystal structure of Acinetobacter baumannii CarO1==
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<StructureSection load='4rl9' size='340' side='right' caption='[[4rl9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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<StructureSection load='4rl9' size='340' side='right'caption='[[4rl9]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rl9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RL9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RL9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rl9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RL9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RL9 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fuv|4fuv]], [[4rlb|4rlb]], [[4rlc|4rlc]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rl9 OCA], [http://pdbe.org/4rl9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rl9 RCSB], [http://www.ebi.ac.uk/pdbsum/4rl9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rl9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rl9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rl9 OCA], [https://pdbe.org/4rl9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rl9 RCSB], [https://www.ebi.ac.uk/pdbsum/4rl9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rl9 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A6XB80_ACIBA A6XB80_ACIBA]
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Outer membrane (OM) beta-barrel proteins composed of 12-18 beta-strands mediate cellular entry of small molecules in Gram-negative bacteria. Small OM proteins with barrels of 10 strands or less are not known to transport small molecules. CarO (carbapenem-associated outer membrane protein) from Acinetobacter baumannii is a small OM protein that has been implicated in the uptake of ornithine and carbapenem antibiotics. Here we report crystal structures of three isoforms of CarO. The structures are very similar and show a monomeric eight-stranded barrel lacking an open channel. CarO has a substantial extracellular domain resembling a glove that contains all the divergent residues between the different isoforms. Liposome swelling experiments demonstrate that full-length CarO and a "loop-less" truncation mutant mediate small-molecule uptake at low levels but that they are unlikely to mediate passage of carbapenem antibiotics. These results are confirmed by biased molecular dynamics simulations that allowed us to quantitatively model the transport of selected small molecules.
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Small-Molecule Transport by CarO, an Abundant Eight-Stranded beta-Barrel Outer Membrane Protein from Acinetobacterbaumannii.,Zahn M, D'Agostino T, Eren E, Basle A, Ceccarelli M, van den Berg B J Mol Biol. 2015 Apr 3. pii: S0022-2836(15)00201-6. doi:, 10.1016/j.jmb.2015.03.016. PMID:25846137<ref>PMID:25846137</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4rl9" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Basle, A]]
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[[Category: Acinetobacter baumannii]]
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[[Category: Berg, B van den]]
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[[Category: Large Structures]]
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[[Category: Zahn, M]]
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[[Category: Basle A]]
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[[Category: Beta-barrel]]
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[[Category: Zahn M]]
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[[Category: Membrane protein]]
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[[Category: Van den Berg B]]
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[[Category: Outer membrane protein]]
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Current revision

Crystal structure of Acinetobacter baumannii CarO1

PDB ID 4rl9

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