1txk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:45, 1 May 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1txk.gif|left|200px]]
 
-
{{Structure
+
==Crystal structure of Escherichia coli OpgG==
-
|PDB= 1txk |SIZE=350|CAPTION= <scene name='initialview01'>1txk</scene>, resolution 2.50&Aring;
+
<StructureSection load='1txk' size='340' side='right'caption='[[1txk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+
<table><tr><td colspan='2'>[[1txk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXK FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
|GENE= opgG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txk OCA], [https://pdbe.org/1txk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txk RCSB], [https://www.ebi.ac.uk/pdbsum/1txk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txk ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txk OCA], [http://www.ebi.ac.uk/pdbsum/1txk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1txk RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/OPGG_ECOLI OPGG_ECOLI] Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).[HAMAP-Rule:MF_01069]
-
 
+
== Evolutionary Conservation ==
-
'''Crystal structure of Escherichia coli OpgG'''
+
[[Image:Consurf_key_small.gif|200px|right]]
-
 
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==Overview==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txk_consurf.spt"</scriptWhenChecked>
-
Osmoregulated periplasmic glucans (OPGs) G protein (OpgG) is required for OPGs biosynthesis. OPGs from Escherichia coli are branched glucans, with a backbone of beta-1,2 glucose units and with branches attached by beta-1,6 linkages. In Proteobacteria, OPGs are involved in osmoprotection, biofilm formation, virulence and resistance to antibiotics. Despite their important biological implications, enzymes synthesizing OPGs are poorly characterized. Here, we report the 2.5 A crystal structure of OpgG from E.coli. The structure was solved using a selenemethionine derivative of OpgG and the multiple anomalous diffraction method (MAD). The protein is composed of two beta-sandwich domains connected by one turn of 3(10) helix. The N-terminal domain (residues 22-388) displays a 25-stranded beta-sandwich fold found in several carbohydrate-related proteins. It exhibits a large cleft comprising many aromatic and acidic residues. This putative binding site shares some similarities with enzymes such as galactose mutarotase and glucodextranase, suggesting a potential catalytic role for this domain in OPG synthesis. On the other hand, the C-terminal domain (residues 401-512) has a seven-stranded immunoglobulin-like beta-sandwich fold, found in many proteins where it is mainly implicated in interactions with other molecules. The structural data suggest that OpgG is an OPG branching enzyme in which the catalytic activity is located in the large N-terminal domain and controlled via the smaller C-terminal domain.
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
1TXK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXK OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txk ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
__TOC__
-
Structural analysis of Escherichia coli OpgG, a protein required for the biosynthesis of osmoregulated periplasmic glucans., Hanoulle X, Rollet E, Clantin B, Landrieu I, Odberg-Ferragut C, Lippens G, Bohin JP, Villeret V, J Mol Biol. 2004 Sep 3;342(1):195-205. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313617 15313617]
+
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bohin, J P.]]
+
[[Category: Bohin JP]]
-
[[Category: Clantin, B.]]
+
[[Category: Clantin B]]
-
[[Category: Hanoulle, X.]]
+
[[Category: Hanoulle X]]
-
[[Category: Landrieu, I.]]
+
[[Category: Landrieu I]]
-
[[Category: Lippens, G.]]
+
[[Category: Lippens G]]
-
[[Category: Odberg-Ferragut, C.]]
+
[[Category: Odberg-Ferragut C]]
-
[[Category: Rollet, E.]]
+
[[Category: Rollet E]]
-
[[Category: Villeret, V.]]
+
[[Category: Villeret V]]
-
[[Category: beta-sandwich]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:02:28 2008''
+

Current revision

Crystal structure of Escherichia coli OpgG

PDB ID 1txk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1txk"
Personal tools