Cellobiohydrolase

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<StructureSection load='3cel' size='350' side='right' scene='48/488493/Cv/1' caption='Glycosylated cellobiohydrolase I catalytic domain complex with cellobiose and Cd+2 ions (yellow) (PDB code [[3cel]])'>
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<StructureSection load='3cel' size='350' side='right' scene='48/488493/Cv/1' caption='Glycosylated cellobiohydrolase I catalytic domain complex with cellobiose and Cd+2 ions (PDB code [[3cel]])'>
== Function ==
== Function ==
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'''Cellobiohydrolase''' (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. There are two types of CBH. '''CBHI''' cleaves progressively from the reducing end while '''CBHII''' cleaves progressively from the nonreducing end of cellulose.<ref>PMID:9466911</ref>
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'''Cellobiohydrolase''' (CBH) is a cellulase which degrades [[cellulose]] by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. There are two types of CBH. '''CBHI''' cleaves progressively from the reducing end while '''CBHII''' cleaves progressively from the nonreducing end of cellulose.<ref>PMID:9466911</ref>. The exo-acting CBH I is called '''Cel7A'''. The endo-acting CBH I is called '''Cel7B'''. '''Cellobiohydrolase Cel6A''' contains an active site within a tunnel which opens and closes in response to ligand binding<ref>PMID:12842048</ref>.
==Structural highlights ==
==Structural highlights ==
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The <scene name='48/488493/Cv/2'>active site</scene> of CBH contains <scene name='48/488493/Cv/3'>Glu-Asp-Glu</scene> (Gln-Asp-Glu in [[3cel]], cyan) and is situated at the <scene name='48/488493/Cv/4'>bottom of a tunnel</scene>.<ref>PMID:8951380</ref>
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The <scene name='48/488493/Cv/6'>active site</scene> of CBH contains <scene name='48/488493/Cv/7'>Glu-Asp-Glu</scene> (Gln-Asp-Glu in [[3cel]], cyan) and is situated at the <scene name='48/488493/Cv/9'>bottom of a tunnel</scene>.<ref>PMID:8951380</ref> Water molecules shown as red spheres.
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</StructureSection>
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==3D structures of cellobiohydrolase==
==3D structures of cellobiohydrolase==
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[[Cellobiohydrolase 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Cellobiohydrolase I
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**[[1cel]] – HjCBHI core – ''Hypocrea jecorina''<BR />
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**[[1cbh]], [[2cbh]] – HjCBHI C terminal – NMR<BR />
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**[[1azh]], [[1az6]], [[1azj]], [[1azk]] - HjCBHI cellulose-binding domain (mutant) - NMR<BR />
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**[[2cel]], [[4cel]], [[1egn]], [[1q2b]] - HjCBHI catalytic domain (mutant) <BR />
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**[[1gpi]] - PcCBHI catalytic domain – ''Phanerochaete chrysosporium''<BR />
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**[[1q9h]], [[3pfj]] - TeCBHI catalytic domain – ''Talaromyces emersonii''<BR />
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**[[2yok]], [[2y9n]] - CBHI catalytic domain – ''Hypocrea lixii''<br />
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**[[5mcc]], [[5mcd]], [[5mce]], [[5mcf]], [[5mch]], [[5mci]], [[5mcj]], [[5mck]], [[5mcl]], [[5mcm]], [[5mcn]], [[4xnn]] – wfCBHI – water flea<br />
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**[[4zzv]], [[5amp]] - GcCBHI catalytic domain – ''Galactomyces candidum''<br />
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*''Cellobiohydrolase I binary complex''
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**[[3cel]] - HjCBHI catalytic domain (mutant) + cellobiose<br />
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**[[5cel]], [[1q2e]] - HjCBHI catalytic domain (mutant) + cellotetraose<br />
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**[[6cel]] - HjCBHI catalytic domain (mutant) + cellopentaose<br />
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**[[7cel]] - HjCBHI catalytic domain (mutant) + glucose<br />
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**[[2v3i]], [[2v3r]] - HjCBHI catalytic domain + phenanthrenolol<br />
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**[[1dy4]] - TrCBHI catalytic domain + propranolol – ''Trichoderma reesei''<BR />
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**[[1h46]] - PcCBHI catalytic domain + propranolol<br />
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**[[1z3t]] - PcCBHI catalytic domain + cellobiose<br />
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**[[1z3v]] - PcCBHI catalytic domain + lactose<br />
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**[[1z3w]] - PcCBHI catalytic domain + cellobioimidazole<BR />
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**[[3pfx]] - TeCBHI catalytic domain + cellobiose<br />
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**[[3pfz]] - TeCBHI catalytic domain + cellotetraose + cellobiose<br />
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**[[3pl3]] - TeCBHI catalytic domain + cellopentaose<br />
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**[[4zzu]] - GgCBHI catalytic domain + cellotetraose – ''Galactomyces geotrichum''<br />
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**[[4zzw]] - GgCBHI catalytic domain + cellobiose <br />
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**[[4zzt]] - GcCBHI catalytic domain + cellotraiose <br />
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*Cellobiohydrolase II
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**[[3cbh]] – HjCBHII core<BR />
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**[[1cb2]] – HjCBHII catalytic domain (mutant) <BR />
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**[[1hgw]], [[1hgy]], [[1oc6]] – HjCBHII catalytic domain (mutant)
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*''Cellobiohydrolase II binary complex''
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**[[1bvw]] - HiCBHII catalytic domain + mannose – ''Humicola insolens''<BR />
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**[[2bvw]] - HiCBHII catalytic domain + glucose + cellotetraose<br />
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**[[1gz1]], [[1oc5]] - HiCBHII catalytic domain (mutant) + cellotetraose<br />
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**[[1oc7]] - HiCBHII catalytic domain (mutant) + cellopentaose<br />
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**[[1ocj]] - HiCBHII catalytic domain + cellopentaose<br />
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**[[1ocb]] - HiCBHII catalytic domain + fluorescent substrate<BR />
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**[[1ocn]] - HiCBHII catalytic domain (mutant) + isofagomine<BR />
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**[[1qjw]] - TrCBHII catalytic domain (mutant) + cellotetraose<br />
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**[[1qk0]] - TrCBHII catalytic domain + polysaccharide<BR />
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**[[1qk2]] - TrCBHII catalytic domain + cellotetraose<br />
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*Other cellobiohydrolases
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**[[1l1y]] – CtCBH – ''Clostridium thermocellum''<BR />
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**[[1rq5]] - CtCBH (mutant) <BR />
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**[[1ut9]] - CtCBH catalytic domain<BR />
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**[[1sqj]] - GgCBH <BR />
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**[[3a64]], [[3vog]] - CcCBH catalytic domain - ''Coprinopsis cinerea''<BR />
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**[[3vof]], [[3voj]] - CcCBHI catalytic domain (mutant)<br />
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**[[4v1z]] – AfCBH 7A – ''Aspergillus fumigatus''<br />
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*''Other cellobiohydrolases binary complex''
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**[[1l2a]] - CtCBH + glucose<br />
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**[[2ebs]] – GgCBH (mutant) + heptasaccharide<BR />
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**[[3a9b]], [[3voh]] - CcCBHI catalytic domain + cellobiose<br />
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**[[3abx]], [[3voi]] - CcCBHI catalytic domain + cellotriose<br />
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**[[4v20]] - AfCBH 7A + disaccharide<br />
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**[[4v0z]] - TrCBH Cel7A + cellobiose<br />
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**[[4vwt]] - TrCBH Cel7A (mutant) + cellobiose<br />
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Glycosylated cellobiohydrolase I catalytic domain complex with cellobiose and Cd+2 ions (PDB code 3cel)

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References

  1. ↑ Divne C, Stahlberg J, Teeri TT, Jones TA. High-resolution crystal structures reveal how a cellulose chain is bound in the 50 A long tunnel of cellobiohydrolase I from Trichoderma reesei. J Mol Biol. 1998 Jan 16;275(2):309-25. PMID:9466911 doi:http://dx.doi.org/10.1006/jmbi.1997.1437
  2. ↑ Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schulein M, Davies GJ. Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens. Structure. 2003 Jul;11(7):855-64. PMID:12842048
  3. ↑ Stahlberg J, Divne C, Koivula A, Piens K, Claeyssens M, Teeri TT, Jones TA. Activity studies and crystal structures of catalytically deficient mutants of cellobiohydrolase I from Trichoderma reesei. J Mol Biol. 1996 Nov 29;264(2):337-49. PMID:8951380 doi:http://dx.doi.org/10.1006/jmbi.1996.0644

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Michal Harel, Alexander Berchansky, Karsten Theis

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