User:Kaitlyn Enderle/Sandbox 1

<StructureSection load='2cI3' size='340' side='right' caption='Dimethylarginine Dimethylaminohydrolase' scene='75/752351/Ddah_loop/2'>

<scene name='75/752351/Ddah_loop/2'>lid region</scene>

DDAH in complex with <scene name='75/752351/Ddah_with_l-citrulline/4'>L-citrulline</scene>

DDAH in complex with <scene name='75/752351/Ddah_l-homocysteine/1'>L-Homocysteine</scene>

DDAH in complex with <scene name='75/752351/Ddah_s-nitroso-l-homocysteine/1'>S-nitroso-L-homocysteine</scene>

DDAH with salt bridge shown <scene name='75/752351/Ddah_salt_bridge/1'>salt bridge</scene>

DDAH with water pore shown <scene name='75/752351/Ddah_water_pore/2'>water pore</scene>

DDAH in complex with <scene name='75/752351/Ddah_with_l-citrulline/3'>L-citrulline</scene>

== Disease ==

== Relevance ==

== Structural highlights ==

==Inhibitors==

===Zinc at pH 6.3===

===Zinc at pH 9===

===L-citrulline, L-homocysteine, and S-nitroso-L-homocysteine===

L-homocysteine and L-citrulline bind in the active site in the same orientation to create the same intermolecular bonds between it and DDAH. L-citrulline is a product of DDAH hydrolyzing ADMA and MMA, suggesting DDAH activity creates a negative feedback look on itself. Both molecules enter the active site and cause DDAH to be in its closed lid formation. The αC on either molecule creates three salt bridges with DDAH: two with the guanidine group of Arg144 and one with the guanidine group Arg97. The molecules are stabilized in the active site by H-bonds: αC-amino group of the ligand to main chain carbonyls of Val267 and Leu29. Side chains of Asp78 and Glu77 with the ureido group of L-citrulline

Like L-homocysteine and L-citrulline, S-nitroso-L-homocysteine binds and the lid region of DDAH is closed. When DDAH reacts with S-nitroso-L-homocysteine, a covalent product, N-thiosulfximide exist in the active site. N-thiosulfximide is stabilized by several salt bridges and H-bonds. Arg144 and Arg97 stabilize the αC-carbonyl group via salt bridges, and Leu29, Val267, and Asp72 stabilize the Cα-amino group.

This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

</StructureSection>

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