1tzo

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[[Image:1tzo.gif|left|200px]]
 
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{{Structure
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==Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore==
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|PDB= 1tzo |SIZE=350|CAPTION= <scene name='initialview01'>1tzo</scene>, resolution 3.60&Aring;
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<StructureSection load='1tzo' size='340' side='right'caption='[[1tzo]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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<table><tr><td colspan='2'>[[1tzo]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._A2012 Bacillus anthracis str. A2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZO FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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|GENE= pagA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzo OCA], [https://pdbe.org/1tzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzo RCSB], [https://www.ebi.ac.uk/pdbsum/1tzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzo ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1sht|1SHT]], [[1tzn|1TZN]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzo OCA], [http://www.ebi.ac.uk/pdbsum/1tzo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tzo RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/PAG_BACAN PAG_BACAN] One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax.
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== Evolutionary Conservation ==
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'''Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzo_consurf.spt"</scriptWhenChecked>
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After binding to cellular receptors and proteolytic activation, the protective antigen component of anthrax toxin forms a heptameric prepore. The prepore later undergoes pH-dependent conversion to a pore, mediating translocation of the edema and lethal factors to the cytosol. We describe structures of the prepore (3.6 A) and a prepore:receptor complex (4.3 A) that reveal the location of pore-forming loops and an unexpected interaction of the receptor with the pore-forming domain. Lower pH is required for prepore-to-pore conversion in the presence of the receptor, indicating that this interaction regulates pH-dependent pore formation. We present an example of a receptor negatively regulating pH-dependent membrane insertion.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1TZO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZO OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzo ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure of heptameric protective antigen bound to an anthrax toxin receptor: a role for receptor in pH-dependent pore formation., Lacy DB, Wigelsworth DJ, Melnyk RA, Harrison SC, Collier RJ, Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13147-51. Epub 2004 Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15326297 15326297]
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</StructureSection>
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[[Category: Bacillus anthracis]]
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[[Category: Bacillus anthracis str. A2012]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Collier, R J.]]
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[[Category: Collier RJ]]
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[[Category: Lacy, D B.]]
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[[Category: Lacy DB]]
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[[Category: Melnyk, R A.]]
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[[Category: Melnyk RA]]
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[[Category: Wigelsworth, D J.]]
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[[Category: Wigelsworth DJ]]
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[[Category: heptamer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:20 2008''
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Current revision

Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore

PDB ID 1tzo

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