1tzy

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[[Image:1tzy.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution==
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|PDB= 1tzy |SIZE=350|CAPTION= <scene name='initialview01'>1tzy</scene>, resolution 1.90&Aring;
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<StructureSection load='1tzy' size='340' side='right'caption='[[1tzy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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<table><tr><td colspan='2'>[[1tzy]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TZY FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzy OCA], [https://pdbe.org/1tzy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tzy RCSB], [https://www.ebi.ac.uk/pdbsum/1tzy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tzy ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1hq3|1hq3]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzy OCA], [http://www.ebi.ac.uk/pdbsum/1tzy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tzy RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/H2A4_CHICK H2A4_CHICK] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tz/1tzy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tzy ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution'''
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==See Also==
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*[[Histone 3D structures|Histone 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle.
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==About this Structure==
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1TZY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TZY OCA].
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==Reference==
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High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511091 16511091]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Baldwin, J P.]]
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[[Category: Baldwin JP]]
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[[Category: Chantalat, L.]]
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[[Category: Chantalat L]]
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[[Category: Lambert, S J.]]
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[[Category: Lambert SJ]]
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[[Category: Nicholson, J M.]]
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[[Category: Nicholson JM]]
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[[Category: Reynolds, C D.]]
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[[Category: Reynolds CD]]
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[[Category: Wood, C M.]]
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[[Category: Wood CM]]
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[[Category: histone-fold]]
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[[Category: tetramer-dimer-dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:29 2008''
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Current revision

Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution

PDB ID 1tzy

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