1u0i

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[[Image:1u0i.jpg|left|200px]]
 
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{{Structure
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==IAAL-E3/K3 heterodimer==
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|PDB= 1u0i |SIZE=350|CAPTION= <scene name='initialview01'>1u0i</scene>
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<StructureSection load='1u0i' size='340' side='right'caption='[[1u0i]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1u0i]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0I FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0i OCA], [https://pdbe.org/1u0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0i RCSB], [https://www.ebi.ac.uk/pdbsum/1u0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0i ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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<div style="background-color:#fffaf0;">
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0i OCA], [http://www.ebi.ac.uk/pdbsum/1u0i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u0i RCSB]</span>
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== Publication Abstract from PubMed ==
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}}
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'''IAAL-E3/K3 heterodimer'''
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==Overview==
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The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design.
The NMR solution structure of a highly stable coiled-coil IAAL-E3/K3 has been solved. The E3/K3 coiled-coil is a 42-residue de novo designed coiled-coil comprising three heptad repeats per subunit, stabilized by hydrophobic contacts within the core and electrostatic interactions at the interface crossing the hydrophobic core which direct heterodimer formation. This E3/K3 domain has previously been shown to have high alpha-helical content as well as possessing a low dissociation constant (70 nM). The E3/K3 structure is completely alpha-helical and is an archetypical coiled-coil in solution, as determined using a combination of (1)H-NOE and homology based structural restraints. This structure provides a structural framework for visualizing the important interactions for stability and specificity, which are key to protein engineering applications such as affinity purification and de novo design.
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==About this Structure==
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NMR solution structure of a highly stable de novo heterodimeric coiled-coil.,Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD Biopolymers. 2004 Dec 5;75(5):367-75. PMID:15457434<ref>PMID:15457434</ref>
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1U0I is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0I OCA].
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==Reference==
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NMR solution structure of a highly stable de novo heterodimeric coiled-coil., Lindhout DA, Litowski JR, Mercier P, Hodges RS, Sykes BD, Biopolymers. 2004 Dec 5;75(5):367-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15457434 15457434]
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[[Category: Protein complex]]
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[[Category: Hodges, R S.]]
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[[Category: Lindhout, D A.]]
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[[Category: Litowski, J R.]]
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[[Category: Mercier, P.]]
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[[Category: Sykes, B D.]]
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[[Category: coiled-coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:41 2008''
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1u0i" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Hodges RS]]
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[[Category: Lindhout DA]]
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[[Category: Litowski JR]]
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[[Category: Mercier P]]
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[[Category: Sykes BD]]

Current revision

IAAL-E3/K3 heterodimer

PDB ID 1u0i

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