5nf8

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'''Unreleased structure'''
 
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The entry 5nf8 is ON HOLD
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==Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation==
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<StructureSection load='5nf8' size='340' side='right'caption='[[5nf8]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5nf8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NF8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nf8 OCA], [https://pdbe.org/5nf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nf8 RCSB], [https://www.ebi.ac.uk/pdbsum/5nf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nf8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCF1_YEAST RCF1_YEAST] Assembly factor that plays a role in the assembly of the respiratory chain supercomplexes (SCs) composed of ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV). Involved in the recruitment of COX13 and RCF2 into cytochrome c oxidase. May also be required for late-stage assembly of the COX12 and COX13 subunits (PubMed:22310663, PubMed:22342701, PubMed:22405070). Required for the generation and maintenance of a normal proton motive force (PMF) across the inner mitochondrial membrane (IMM) by preventing proton leakage through an inactive population of CIV that accumulates when RCF1 and/or RCF2 proteins are absent (PubMed:30683696, PubMed:31591265).<ref>PMID:22310663</ref> <ref>PMID:22342701</ref> <ref>PMID:22405070</ref> <ref>PMID:30683696</ref> <ref>PMID:31591265</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Saccharomyces cerevisiae respiratory supercomplex factor 1 (Rcf1) protein is located in the mitochondrial inner membrane where it is involved in formation of supercomplexes composed of respiratory complexes III and IV. We report the solution structure of Rcf1, which forms a dimer in dodecylphosphocholine (DPC) micelles, where each monomer consists of a bundle of five transmembrane (TM) helices and a short flexible soluble helix (SH). Three TM helices are unusually charged and provide the dimerization interface consisting of 10 putative salt bridges, defining a "charge zipper" motif. The dimer structure is supported by molecular dynamics (MD) simulations in DPC, although the simulations show a more dynamic dimer interface than the NMR data. Furthermore, CD and NMR data indicate that Rcf1 undergoes a structural change when reconstituted in liposomes, which is supported by MD data, suggesting that the dimer structure is unstable in a planar membrane environment. Collectively, these data indicate a dynamic monomer-dimer equilibrium. Furthermore, the Rcf1 dimer interacts with cytochrome c, suggesting a role as an electron-transfer bridge between complexes III and IV. The Rcf1 structure will help in understanding its functional roles at a molecular level.
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Authors: Zhou, S., Pettersson, P., Sjoholm, J., Sjostrand, D., Hogbom, M., Brzezinski, P., Maler, L., Adelroth, P.
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Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation.,Zhou S, Pettersson P, Huang J, Sjoholm J, Sjostrand D, Pomes R, Hogbom M, Brzezinski P, Maler L, Adelroth P Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 1712061115. doi:, 10.1073/pnas.1712061115. PMID:29507228<ref>PMID:29507228</ref>
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Description: Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Hogbom, M]]
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<div class="pdbe-citations 5nf8" style="background-color:#fffaf0;"></div>
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[[Category: Sjostrand, D]]
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== References ==
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[[Category: Maler, L]]
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<references/>
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[[Category: Brzezinski, P]]
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__TOC__
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[[Category: Adelroth, P]]
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</StructureSection>
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[[Category: Zhou, S]]
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[[Category: Large Structures]]
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[[Category: Sjoholm, J]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Pettersson, P]]
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[[Category: Adelroth P]]
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[[Category: Brzezinski P]]
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[[Category: Hogbom M]]
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[[Category: Maler L]]
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[[Category: Pettersson P]]
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[[Category: Sjoholm J]]
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[[Category: Sjostrand D]]
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[[Category: Zhou S]]

Current revision

Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation

PDB ID 5nf8

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