5nhg

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(New page: '''Unreleased structure''' The entry 5nhg is ON HOLD Authors: Szabo, E., Mizsei, R., Wilk, P., Zambo, Z., Torocsik, B., Weiss, M.S., Adam-Vizi, V., Ambrus, A. Description: Crystal stru...)
Current revision (12:58, 15 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5nhg is ON HOLD
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==Crystal structure of the human dihydrolipoamide dehydrogenase==
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<StructureSection load='5nhg' size='340' side='right'caption='[[5nhg]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5nhg]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NHG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nhg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nhg OCA], [https://pdbe.org/5nhg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nhg RCSB], [https://www.ebi.ac.uk/pdbsum/5nhg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nhg ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/DLDH_HUMAN DLDH_HUMAN] Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis. Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:[https://omim.org/entry/248600 248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation.
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== Function ==
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[https://www.uniprot.org/uniprot/DLDH_HUMAN DLDH_HUMAN] Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report the crystal structures of the human (dihydro)lipoamide dehydrogenase (hLADH, hE3) and its disease-causing homodimer interface mutant D444V-hE3 at 2.27 and 1.84A resolution, respectively. The wild type structure is a unique uncomplexed, unliganded hE3 structure with the true canonical sequence. Based on the structural information a novel molecular pathomechanism is proposed for the impaired catalytic activity and enhanced capacity for reactive oxygen species generation of the pathogenic mutant. The mechanistic model involves a previously much ignored solvent accessible channel leading to the active site that might be perturbed also by other disease-causing homodimer interface substitutions of this enzyme.
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Authors: Szabo, E., Mizsei, R., Wilk, P., Zambo, Z., Torocsik, B., Weiss, M.S., Adam-Vizi, V., Ambrus, A.
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Crystal structures of the disease-causing D444V mutant and the relevant wild type human dihydrolipoamide dehydrogenase.,Szabo E, Mizsei R, Wilk P, Zambo Z, Torocsik B, Weiss MS, Adam-Vizi V, Ambrus A Free Radic Biol Med. 2018 Jun 20;124:214-220. doi:, 10.1016/j.freeradbiomed.2018.06.008. PMID:29908278<ref>PMID:29908278</ref>
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Description: Crystal structure of the human dihydrolipoamide dehydrogenase
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Ambrus, A]]
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<div class="pdbe-citations 5nhg" style="background-color:#fffaf0;"></div>
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[[Category: Zambo, Z]]
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== References ==
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[[Category: Wilk, P]]
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<references/>
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[[Category: Adam-Vizi, V]]
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__TOC__
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[[Category: Szabo, E]]
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</StructureSection>
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[[Category: Mizsei, R]]
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[[Category: Homo sapiens]]
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[[Category: Torocsik, B]]
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[[Category: Large Structures]]
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[[Category: Weiss, M.S]]
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[[Category: Adam-Vizi V]]
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[[Category: Ambrus A]]
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[[Category: Mizsei R]]
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[[Category: Szabo E]]
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[[Category: Torocsik B]]
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[[Category: Weiss MS]]
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[[Category: Wilk P]]
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[[Category: Zambo Z]]

Current revision

Crystal structure of the human dihydrolipoamide dehydrogenase

PDB ID 5nhg

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