5v5s

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'''Unreleased structure'''
 
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The entry 5v5s is ON HOLD
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==multi-drug efflux; membrane transport; RND superfamily; Drug resistance==
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<SX load='5v5s' size='340' side='right' viewer='molstar' caption='[[5v5s]], [[Resolution|resolution]] 6.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5v5s]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V5S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v5s OCA], [https://pdbe.org/5v5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v5s RCSB], [https://www.ebi.ac.uk/pdbsum/5v5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v5s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TOLC_ECOLI TOLC_ECOLI] Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.<ref>PMID:6337123</ref> <ref>PMID:11274125</ref> <ref>PMID:15228545</ref> <ref>PMID:18955484</ref> <ref>PMID:23176499</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacterial efflux pumps confer multidrug resistance by transporting diverse antibiotics from the cell. In Gram-negative bacteria, some of these pumps form multi-protein assemblies that span the cell envelope. Here we report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that allosterically couples and synchronizes initial ligand binding with channel opening. Within the transport-activated state, the channel remains open even though the pump cycles through three distinct conformations. Collectively, our data provide a dynamic mechanism for the assembly and operation of the AcrAB-TolC pump.
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Authors: wang, Z., fan, G., Hryc, C.F., Blaza, J.N., Serysheva, I.I., Schmid, M.F., Chiu, W., Luisi, B.F., Du, D.
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An allosteric transport mechanism for the AcrAB-TolC Multidrug Efflux Pump.,Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D Elife. 2017 Mar 29;6. pii: e24905. doi: 10.7554/eLife.24905. PMID:28355133<ref>PMID:28355133</ref>
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Description: multi-drug efflux; membrane transport; RND superfamily; Drug resistance
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Du, D]]
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<div class="pdbe-citations 5v5s" style="background-color:#fffaf0;"></div>
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[[Category: Luisi, B.F]]
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== References ==
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[[Category: Fan, G]]
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<references/>
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[[Category: Chiu, W]]
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__TOC__
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[[Category: Blaza, J.N]]
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</SX>
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[[Category: Hryc, C.F]]
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[[Category: Escherichia coli]]
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[[Category: Schmid, M.F]]
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[[Category: Large Structures]]
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[[Category: Serysheva, I.I]]
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[[Category: Blaza JN]]
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[[Category: Wang, Z]]
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[[Category: Chiu W]]
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[[Category: Du D]]
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[[Category: Hryc CF]]
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[[Category: Luisi BF]]
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[[Category: Schmid MF]]
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[[Category: Serysheva II]]
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[[Category: Fan G]]
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[[Category: Wang Z]]

Current revision

multi-drug efflux; membrane transport; RND superfamily; Drug resistance

5v5s, resolution 6.50Å

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OCA

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