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5xam

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(New page: '''Unreleased structure''' The entry 5xam is ON HOLD Authors: Tsukazaki, T., Tanaka, Y., Furukwa, A. Description: Crystal structure of the protein secretion motor [[Category: Unrelease...)
Current revision (07:58, 22 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 5xam is ON HOLD
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==Crystal structure of SecDF in I form at 4 A resolution==
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<StructureSection load='5xam' size='340' side='right'caption='[[5xam]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5xam]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XAM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xam FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xam OCA], [https://pdbe.org/5xam PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xam RCSB], [https://www.ebi.ac.uk/pdbsum/5xam PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xam ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9RTE3_DEIRA Q9RTE3_DEIRA] Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.[HAMAP-Rule:MF_01464][SAAS:SAAS00541769]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein secretion mediated by SecYEG translocon and SecA ATPase is enhanced by membrane-embedded SecDF by using proton motive force. A previous structural study of SecDF indicated that it comprises 12 transmembrane helices that can conduct protons and three periplasmic domains, which form at least two characterized transition states, termed the F and I forms. We report the structures of full-length SecDF in I form at 2.6- to 2.8-A resolution. The structures revealed that SecDF in I form can generate a tunnel that penetrates the transmembrane region and functions as a proton pathway regulated by a conserved Asp residue of the transmembrane region. In one crystal structure, periplasmic cavity interacts with a molecule, potentially polyethylene glycol, which may mimic a substrate peptide. This study provides structural insights into the Sec protein translocation that allows future analyses to develop a more detailed working model for SecDF.
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Authors: Tsukazaki, T., Tanaka, Y., Furukwa, A.
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Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF.,Furukawa A, Yoshikaie K, Mori T, Mori H, Morimoto YV, Sugano Y, Iwaki S, Minamino T, Sugita Y, Tanaka Y, Tsukazaki T Cell Rep. 2017 May 2;19(5):895-901. doi: 10.1016/j.celrep.2017.04.030. PMID:28467902<ref>PMID:28467902</ref>
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Description: Crystal structure of the protein secretion motor
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Tsukazaki, T]]
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<div class="pdbe-citations 5xam" style="background-color:#fffaf0;"></div>
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[[Category: Tanaka, Y]]
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== References ==
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[[Category: Furukwa, A]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Deinococcus radiodurans R1]]
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[[Category: Large Structures]]
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[[Category: Furukwa A]]
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[[Category: Tanaka Y]]
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[[Category: Tsukazaki T]]

Current revision

Crystal structure of SecDF in I form at 4 A resolution

PDB ID 5xam

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