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1u8x

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CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE

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Retrieved from "http://52.214.119.220/wiki/index.php/1u8x"

@@ Line 1: / Line 1: @@
-[[Image:1u8x.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE==
-|PDB= 1u8x |SIZE=350|CAPTION= <scene name='initialview01'>1u8x</scene>, resolution 2.05&Aring;
+<StructureSection load='1u8x' size='340' side='right'caption='[[1u8x]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
+<table><tr><td colspan='2'>[[1u8x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nrh 1nrh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U8X FirstGlance]. <br>
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Maltose-6'-phosphate_glucosidase Maltose-6'-phosphate glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.122 3.2.1.122] </span>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-|GENE= glvA, glvG, glv-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam02056 Glyco_hydro_4]</span>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8x OCA], [https://pdbe.org/1u8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u8x RCSB], [https://www.ebi.ac.uk/pdbsum/1u8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u8x ProSAT], [https://www.topsan.org/Proteins/MCSG/1u8x TOPSAN]</span></td></tr>
-|RELATEDENTRY=[[1nrh|1NRH]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8x OCA], [http://www.ebi.ac.uk/pdbsum/1u8x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u8x RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/GLVA_BACSU GLVA_BACSU] Hydrolyzes maltose-6'-phosphate and trehalose-6'-phosphate. Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system (PEP-PTS). Is also able to significantly catalyze the hydrolysis of both 6-phospho-alpha- and 6-phospho-beta-glucosides containing activated leaving groups such as p-nitrophenol and does so with retention and inversion, respectively, of the substrate anomeric configuration.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u8/1u8x_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u8x ConSurf].
+<div style="clear:both"></div>
-'''CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE'''
+==See Also==
+*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-GlvA, a 6-phospho-alpha-glucosidase from Bacillus subtilis, catalyzes the hydrolysis of maltose-6'-phosphate and belongs to glycoside hydrolase family GH4. GH4 enzymes are unique in their requirement for NAD(H) and a divalent metal for activity. We have determined the crystal structure of GlvA in complex with its ligands to 2.05 A resolution. Analyses of the active site architecture, in conjunction with mechanistic studies and precedent from the nucleotide diphosphate hexose dehydratases and other systems, suggest a novel mechanism of glycoside hydrolysis by GlvA that involves both the NAD(H) and the metal.
-==About this Structure==
-U8X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry 1NRH. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8X OCA].
-==Reference==
-Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis., Rajan SS, Yang X, Collart F, Yip VL, Withers SG, Varrot A, Thompson J, Davies GJ, Anderson WF, Structure. 2004 Sep;12(9):1619-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15341727 15341727]
 [[Category: Bacillus subtilis]]
-[[Category: Maltose-6'-phosphate glucosidase]]
+[[Category: Large Structures]]
-[[Category: Single protein]]
+[[Category: Anderson WF]]
-[[Category: Anderson, W F.]]
+[[Category: Collart F]]
-[[Category: Collart, F.]]
+[[Category: Rajan SS]]
-[[Category: MCSG, Midwest Center for Structural Genomics.]]
+[[Category: Yang X]]
-[[Category: Rajan, S S.]]
-[[Category: Yang, X.]]
-[[Category: glucosidase]]
-[[Category: mcsg]]
-[[Category: midwest center for structural genomic]]
-[[Category: nad-dependent]]
-[[Category: protein structure initiative]]
-[[Category: psi]]
-[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:06:57 2008''

1u8x

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CRYSTAL STRUCTURE OF GLVA FROM BACILLUS SUBTILIS, A METAL-REQUIRING, NAD-DEPENDENT 6-PHOSPHO-ALPHA-GLUCOSIDASE

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Function

Evolutionary Conservation

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