5k7p

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==MicroED structure of xylanase at 2.3 A resolution==
==MicroED structure of xylanase at 2.3 A resolution==
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<StructureSection load='5k7p' size='340' side='right' caption='[[5k7p]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='5k7p' size='340' side='right'caption='[[5k7p]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5k7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K7P FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5k7p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_reesei Trichoderma reesei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K7P FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k7n|5k7n]], [[5k7o|5k7o]], [[5k7q|5k7q]], [[5k7r|5k7r]], [[5k7s|5k7s]], [[5k7t|5k7t]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7p OCA], [https://pdbe.org/5k7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k7p RCSB], [https://www.ebi.ac.uk/pdbsum/5k7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7p ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k7p OCA], [http://pdbe.org/5k7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k7p RCSB], [http://www.ebi.ac.uk/pdbsum/5k7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k7p ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/XYN2_HYPJR XYN2_HYPJR]] Glycoside hydrolase involved in the hydrolysis of xylan, a major plant cell wall hemicellulose made up of 1,4-beta-linked D-xylopyranose residues. Catalyzes the endohydrolysis of the main-chain 1,4-beta-glycosidic bonds connecting the xylose subunits yielding various xylooligosaccharides and xylose (PubMed:1369024, Ref.5). The catalysis proceeds by a double-displacement reaction mechanism with a putative covalent glycosyl-enzyme intermediate, with retention of the anomeric configuration (PubMed:7988708). Produces xylobiose and xylose as the main degradation products (PubMed:19556747).<ref>PMID:1369024</ref> <ref>PMID:19556747</ref> <ref>PMID:7988708</ref> <ref>PMID:1369024</ref>
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[https://www.uniprot.org/uniprot/XYN2_HYPJR XYN2_HYPJR] Glycoside hydrolase involved in the hydrolysis of xylan, a major plant cell wall hemicellulose made up of 1,4-beta-linked D-xylopyranose residues. Catalyzes the endohydrolysis of the main-chain 1,4-beta-glycosidic bonds connecting the xylose subunits yielding various xylooligosaccharides and xylose (PubMed:1369024, Ref.5). The catalysis proceeds by a double-displacement reaction mechanism with a putative covalent glycosyl-enzyme intermediate, with retention of the anomeric configuration (PubMed:7988708). Produces xylobiose and xylose as the main degradation products (PubMed:19556747).<ref>PMID:1369024</ref> <ref>PMID:19556747</ref> <ref>PMID:7988708</ref> <ref>PMID:1369024</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.
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Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.,de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T Nat Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178. PMID:28192420<ref>PMID:28192420</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5k7p" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Large Structures]]
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[[Category: Hypocrea jecorina]]
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[[Category: Trichoderma reesei]]
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[[Category: Cascio, D]]
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[[Category: Cascio D]]
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[[Category: Cruz, M J.de la]]
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[[Category: Eisenberg D]]
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[[Category: Eisenberg, D]]
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[[Category: Gonen T]]
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[[Category: Gonen, T]]
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[[Category: Hattne J]]
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[[Category: Hattne, J]]
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[[Category: Reyes FE]]
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[[Category: Reyes, F E]]
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[[Category: Rodriguez J]]
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[[Category: Rodriguez, J]]
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[[Category: Sawaya MR]]
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[[Category: Sawaya, M R]]
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[[Category: Seidler P]]
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[[Category: Seidler, P]]
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[[Category: Shi D]]
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[[Category: Shi, D]]
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[[Category: De la Cruz MJ]]
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[[Category: Hydrolase]]
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Current revision

MicroED structure of xylanase at 2.3 A resolution

PDB ID 5k7p

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