5nbz

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==Wzz dodecamer fitted by MDFF to the Wzz experimental map from cryo-EM==
==Wzz dodecamer fitted by MDFF to the Wzz experimental map from cryo-EM==
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<StructureSection load='5nbz' size='340' side='right' caption='[[5nbz]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
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<SX load='5nbz' size='340' side='right' viewer='molstar' caption='[[5nbz]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5nbz]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NBZ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5nbz]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NBZ FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbz OCA], [http://pdbe.org/5nbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nbz RCSB], [http://www.ebi.ac.uk/pdbsum/5nbz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbz ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbz OCA], [https://pdbe.org/5nbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nbz RCSB], [https://www.ebi.ac.uk/pdbsum/5nbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbz ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/WZZB3_ECOLX WZZB3_ECOLX] Confers a modal distribution of chain length on the O-antigen component of lipopolysaccharide (LPS). Gives rise to a reduced number of short chain molecules and increases in numbers of longer molecules, with a modal value of 13 (in strain O111/M92) and of 17 (in strain K12).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Wzz is an integral inner membrane protein involved in regulating the length of lipopolysaccharide O-antigen glycans and essential for the virulence of many Gram-negative pathogens. In all Wzz homologs, the large periplasmic domain is proposed to be anchored by two transmembrane helices, but no information is available for the transmembrane and cytosolic domains. Here we have studied purified oligomeric Wzz complexes using cryoelectron microscopy and resolved the transmembrane regions within a semi-continuous detergent micelle. The transmembrane helices of each monomer display a right-handed super-helical twist, and do not interact with the neighboring transmembrane domains. Modeling, flexible fitting and multiscale simulation approaches were used to study the full-length complex and to provide explanations for the influence of the lipid bilayer on its oligomeric status. Based on structural and in silico observations, we propose a new mechanism for O-antigen chain-length regulation that invokes synergy of Wzz and its polymerase partner, Wzy.
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Full-length, Oligomeric Structure of Wzz Determined by Cryoelectron Microscopy Reveals Insights into Membrane-Bound States.,Collins RF, Kargas V, Clarke BR, Siebert CA, Clare DK, Bond PJ, Whitfield C, Ford RC Structure. 2017 May 2;25(5):806-815.e3. doi: 10.1016/j.str.2017.03.017. Epub 2017, Apr 20. PMID:28434914<ref>PMID:28434914</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5nbz" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Bond, P J]]
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[[Category: Large Structures]]
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[[Category: Clare, D K]]
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[[Category: Staphylococcus aureus]]
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[[Category: Clarke, B R]]
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[[Category: Bond PJ]]
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[[Category: Collins, R F]]
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[[Category: Clare DK]]
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[[Category: Ford, R C]]
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[[Category: Clarke BR]]
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[[Category: Kargas, V]]
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[[Category: Collins RF]]
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[[Category: Siebert, A]]
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[[Category: Ford RC]]
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[[Category: Whitfield, C]]
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[[Category: Kargas V]]
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[[Category: Membrane protein]]
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[[Category: Siebert A]]
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[[Category: Wzz polysaccharide chain length membrane protein cryo-electron microscopy single particle analysis]]
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[[Category: Whitfield C]]

Current revision

Wzz dodecamer fitted by MDFF to the Wzz experimental map from cryo-EM

5nbz, resolution 9.00Å

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