1u9t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:44, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1u9t.gif|left|200px]]
 
-
{{Structure
+
==Crystal Structure Analysis of ChuS, an E. coli Heme Oxygenase==
-
|PDB= 1u9t |SIZE=350|CAPTION= <scene name='initialview01'>1u9t</scene>, resolution 2.16&Aring;
+
<StructureSection load='1u9t' size='340' side='right'caption='[[1u9t]], [[Resolution|resolution]] 2.16&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1u9t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7_str._EDL933 Escherichia coli O157:H7 str. EDL933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9T FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16&#8491;</td></tr>
-
|GENE= ChuS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9t OCA], [https://pdbe.org/1u9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9t RCSB], [https://www.ebi.ac.uk/pdbsum/1u9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9t ProSAT], [https://www.topsan.org/Proteins/BSGI/1u9t TOPSAN]</span></td></tr>
-
|DOMAIN=
+
</table>
-
|RELATEDENTRY=
+
== Function ==
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9t OCA], [http://www.ebi.ac.uk/pdbsum/1u9t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u9t RCSB]</span>
+
[https://www.uniprot.org/uniprot/Q8X5N8_ECO57 Q8X5N8_ECO57]
-
}}
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
'''Crystal Structure Analysis of ChuS, an E. coli Heme Oxygenase'''
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u9t_consurf.spt"</scriptWhenChecked>
-
==Overview==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
Heme oxygenases (HOs) catalyze the oxidation of heme to biliverdin, carbon monoxide (CO), and free iron. Iron acquisition is critical for invading microorganisms to enable survival and growth. Here we report the crystal structure of ChuS, which displays a previously uncharacterized fold and is unique compared with other characterized HOs. Despite only 19% sequence identity between the N- and C-terminal halves, these segments of ChuS represent a structural duplication, with a root-mean-square deviation of 2.1 A between the two repeats. ChuS is capable of using ascorbic acid or cytochrome P450 reductase-NADPH as electron sources for heme oxygenation. CO detection confirmed that ChuS is a HO, and we have identified it in pathogenic Escherichia coli O157:H7. Based on sequence analysis, this HO is present in many bacteria, although not in the E. coli K-12 strain. The N- and C-terminal halves of ChuS are each a functional HO.
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==About this Structure==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u9t ConSurf].
-
1U9T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9T OCA].
+
<div style="clear:both"></div>
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Identification of an Escherichia coli O157:H7 heme oxygenase with tandem functional repeats., Suits MD, Pal GP, Nakatsu K, Matte A, Cygler M, Jia Z, Proc Natl Acad Sci U S A. 2005 Nov 22;102(47):16955-60. Epub 2005 Nov 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16275907 16275907]
+
[[Category: Escherichia coli O157:H7 str. EDL933]]
-
[[Category: Escherichia coli]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Jia Z]]
-
[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
+
[[Category: Suits MD]]
-
[[Category: Jia, Z.]]
+
-
[[Category: Suits, M D.]]
+
-
[[Category: bsgi]]
+
-
[[Category: central beta sheet]]
+
-
[[Category: flanked by alpha helice]]
+
-
[[Category: structural genomic]]
+
-
[[Category: structural repeat]]
+
-
[[Category: the montreal-kingston bacterial structural genomics initiative]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:18 2008''
+

Current revision

Crystal Structure Analysis of ChuS, an E. coli Heme Oxygenase

PDB ID 1u9t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u9t"
Personal tools