1ua7

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[[Image:1ua7.gif|left|200px]]
 
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{{Structure
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==Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose==
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|PDB= 1ua7 |SIZE=350|CAPTION= <scene name='initialview01'>1ua7</scene>, resolution 2.21&Aring;
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<StructureSection load='1ua7' size='340' side='right'caption='[[1ua7]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACI:6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL'>ACI</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G6D:6-DEOXY-ALPHA-D-GLUCOSE'>G6D</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=GLD:4,6-DIDEOXYGLUCOSE'>GLD</scene>
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<table><tr><td colspan='2'>[[1ua7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UA7 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACI:6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL'>ACI</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=G6D:6-DEOXY-ALPHA-D-GLUCOSE'>G6D</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GLD:4,6-DIDEOXYGLUCOSE'>GLD</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ua7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ua7 OCA], [https://pdbe.org/1ua7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ua7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ua7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ua7 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1bag|1BAG]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ua7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ua7 OCA], [http://www.ebi.ac.uk/pdbsum/1ua7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ua7 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/AMY_BACSU AMY_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ua/1ua7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ua7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process.
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'''Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose'''
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Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.,Kagawa M, Fujimoto Z, Momma M, Takase K, Mizuno H J Bacteriol. 2003 Dec;185(23):6981-4. PMID:14617662<ref>PMID:14617662</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ua7" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process.
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1UA7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UA7 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose., Kagawa M, Fujimoto Z, Momma M, Takase K, Mizuno H, J Bacteriol. 2003 Dec;185(23):6981-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14617662 14617662]
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[[Category: Alpha-amylase]]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fujimoto, Z.]]
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[[Category: Fujimoto Z]]
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[[Category: Kagawa, M.]]
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[[Category: Kagawa M]]
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[[Category: Mizuno, H.]]
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[[Category: Mizuno H]]
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[[Category: Momma, M.]]
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[[Category: Momma M]]
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[[Category: Takase, K.]]
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[[Category: Takase K]]
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[[Category: acarbose]]
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[[Category: beta-alpha-barrel]]
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[[Category: greek-key motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:28 2008''
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Current revision

Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose

PDB ID 1ua7

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