5nm8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5nm8 is ON HOLD until Paper Publication Authors: Tremino, L., Forcada-Nadal, A., Labella, J.I., Cantos, R., Espinosa, J., Contreras, A., Rubio, V. ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942, with PLP bound== | |
| + | <StructureSection load='5nm8' size='340' side='right'caption='[[5nm8]], [[Resolution|resolution]] 1.93Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5nm8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NM8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nm8 OCA], [https://pdbe.org/5nm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nm8 RCSB], [https://www.ebi.ac.uk/pdbsum/5nm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nm8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q31LH9_SYNE7 Q31LH9_SYNE7] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Synechococcus elongatus COG0325 gene pipY functionally interacts with the nitrogen regulatory gene pipX. As a first step toward a molecular understanding of such interactions, we characterized PipY. This 221-residue protein is monomeric and hosts pyridoxal phosphate (PLP), binding it with limited affinity and losing it upon incubation with D-cycloserine. PipY crystal structures with and without PLP reveal a single-domain monomer folded as the TIM barrel of type-III fold PLP enzymes, with PLP highly exposed, fitting a role for PipY in PLP homeostasis. The mobile PLP phosphate-anchoring C-terminal helix might act as a trigger for PLP exchange. Exploiting the universality of COG0325 functions, we used PipY in site-directed mutagenesis studies to shed light on disease causation by epilepsy-associated mutations in the human COG0325 gene PROSC. | ||
| - | + | Studies on cyanobacterial protein PipY shed light on structure, potential functions, and vitamin B6 -dependent epilepsy.,Tremino L, Forcada-Nadal A, Contreras A, Rubio V FEBS Lett. 2017 Sep 15. doi: 10.1002/1873-3468.12841. PMID:28914444<ref>PMID:28914444</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 5nm8" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Forcada-Nadal | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | + | [[Category: Synechococcus elongatus PCC 7942 = FACHB-805]] | |
| + | [[Category: Contreras A]] | ||
| + | [[Category: Forcada-Nadal A]] | ||
| + | [[Category: Rubio V]] | ||
| + | [[Category: Tremino L]] | ||
Current revision
Structure of PipY, the COG0325 family member of Synechococcus elongatus PCC7942, with PLP bound
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