1ufi

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[[Image:1ufi.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the dimerization domain of human CENP-B==
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|PDB= 1ufi |SIZE=350|CAPTION= <scene name='initialview01'>1ufi</scene>, resolution 1.65&Aring;
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<StructureSection load='1ufi' size='340' side='right'caption='[[1ufi]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1ufi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UFI FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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|GENE= CENPB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufi OCA], [https://pdbe.org/1ufi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ufi RCSB], [https://www.ebi.ac.uk/pdbsum/1ufi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ufi ProSAT], [https://www.topsan.org/Proteins/RSGI/1ufi TOPSAN]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ufi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufi OCA], [http://www.ebi.ac.uk/pdbsum/1ufi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ufi RCSB]</span>
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[https://www.uniprot.org/uniprot/CENPB_HUMAN CENPB_HUMAN] Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
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}}
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human centromere protein B (CENP-B), a centromeric heterochromatin component, forms a homodimer that specifically binds to a distinct DNA sequence (the CENP-B box), which appears within every other alpha-satellite repeat. Previously, we determined the structure of the human CENP-B DNA-binding domain, CENP-B-(1-129), complexed with the CENP-B box DNA. In the present study, we determined the crystal structure of its dimerization domain (CENP-B-(540-599)), another functional domain of CENP-B, at 1.65-A resolution. CENP-B-(540-599) contains two alpha-helices, which are folded into an antiparallel configuration. The CENP-B-(540-599) dimer formed a symmetrical, antiparallel, four-helix bundle structure with a large hydrophobic patch in which 23 residues of one monomer form van der Waals contacts with the other monomer. In the CENP-B-(540-599) dimer, the N-terminal ends of CENP-B-(540-599) are oriented on opposite sides of the dimer. This CENP-B dimer configuration may be suitable for capturing two distant CENP-B boxes during centromeric heterochromatin formation.
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'''Crystal structure of the dimerization domain of human CENP-B'''
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Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution.,Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S J Biol Chem. 2003 Dec 19;278(51):51454-61. Epub 2003 Sep 30. PMID:14522975<ref>PMID:14522975</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ufi" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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The human centromere protein B (CENP-B), a centromeric heterochromatin component, forms a homodimer that specifically binds to a distinct DNA sequence (the CENP-B box), which appears within every other alpha-satellite repeat. Previously, we determined the structure of the human CENP-B DNA-binding domain, CENP-B-(1-129), complexed with the CENP-B box DNA. In the present study, we determined the crystal structure of its dimerization domain (CENP-B-(540-599)), another functional domain of CENP-B, at 1.65-A resolution. CENP-B-(540-599) contains two alpha-helices, which are folded into an antiparallel configuration. The CENP-B-(540-599) dimer formed a symmetrical, antiparallel, four-helix bundle structure with a large hydrophobic patch in which 23 residues of one monomer form van der Waals contacts with the other monomer. In the CENP-B-(540-599) dimer, the N-terminal ends of CENP-B-(540-599) are oriented on opposite sides of the dimer. This CENP-B dimer configuration may be suitable for capturing two distant CENP-B boxes during centromeric heterochromatin formation.
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*[[Centromere protein 3D structure|Centromere protein 3D structure]]
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== References ==
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==About this Structure==
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<references/>
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1UFI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UFI OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Crystal structure of the human centromere protein B (CENP-B) dimerization domain at 1.65-A resolution., Tawaramoto MS, Park SY, Tanaka Y, Nureki O, Kurumizaka H, Yokoyama S, J Biol Chem. 2003 Dec 19;278(51):51454-61. Epub 2003 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14522975 14522975]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kurumizaka, H.]]
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[[Category: Kurumizaka H]]
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[[Category: Park, S Y.]]
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[[Category: Park S-Y]]
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[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
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[[Category: Tanaka Y]]
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[[Category: Tanaka, Y.]]
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[[Category: Tawaramoto MS]]
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[[Category: Tawaramoto, M S.]]
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[[Category: Yokoyama S]]
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[[Category: Yokoyama, S.]]
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[[Category: dimerization domain]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: salt bridge]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:09:34 2008''
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Current revision

Crystal structure of the dimerization domain of human CENP-B

PDB ID 1ufi

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