Sandbox GGC1
From Proteopedia
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| - | == | + | ==Kinesin Motor Domain== |
| - | <StructureSection load=' | + | <StructureSection load='1bg2' size='340' side='right' caption='Kinesin Protein' scene=''> |
| - | + | Kinesin is an ATP-dependent motor protein responsible for the transportation of cargo. It is a heterotetramer composed of light and heavy chains. | |
| + | == '''Function''' == | ||
| + | * Moving cargo usually from the center of the cell to the margins <br/> | ||
| + | * Involved in cell replication and axonal signaling | ||
| - | + | == '''Disease''' == | |
| + | *Charcot Marie Tooth (CMT): Charcot Marie Tooth is one of many diseases that affect the peripheral nervous system. Individuals may suffer physical disabilities due to weakness in transmission of signals firing from the central nervous system | ||
| - | == Function == | ||
| - | The active site of chymotrypsin consists of a <scene name='75/752263/Active_site/3'>catalytic triad</scene> (Ser 195, His 57, Asp 102), which are highlighted in blue. | ||
| - | The S1 binding pocket is responsible for stabilization of the substrate in the active site prior to cleavage. The S1 pocket is mainly hydrophobic and preferentially binds to large, nonpolar amino acids, which includes tyrosine, tryptophan, and phenylalanine. The <scene name='75/752263/Active_site/2'>S1 pocket</scene> of the bovine alpha-chymotrypsin is highlighted in yellow. | ||
| - | Chymotrypsin and other serine protease enzymes catalyzes the cleavage of amino acids, and the S1 binding pocket helps stabilize the intermediate before completely cleaving the amino acid. Both the active site and S1 pocket can be seen <scene name='75/752263/Both_active_site_and_s1/1'>here</scene>. The catalytic triad is highlighted in blue and the S1 pocket is highlighted in yellow. | ||
| - | == Disease == | ||
| - | == | + | == '''Structural highlights''' == |
| - | = | + | ;Scene #1: Kinesin structure is composed of eight anti-parallel Beta sheets and three alpha helices on opposite sides <scene name='75/752263/Alpha_beta_sheets/5'> Alpha-beta structure </scene> |
| - | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/ | + | ;Scene #2: This is the ATP binding site. Upon the binding of phosphate to ADP, ATP is formed giving Kinesin the energy needed to move the cargo one step a head to its destination <scene name='75/752263/Atp_binding_site/5'> ATP-binding site </scene> |
| + | |||
| + | ;Scene #3: This is the Microtubule-binding site, where Kinesin binds to microtubules for stability <scene name='75/752263/Mt-binding_site/1'> MT-binding site </scene> | ||
| + | |||
| + | ;Scene #4: some patients with CMT show mutation in the p-loop. A missense mutation results in a leucine in place of glutamine affecting signal transmission <scene name='75/752263/Cmt/1'> CMT </scene> | ||
| + | |||
| + | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
| + | #file:///C:/Users/Muna/Desktop/Spring%202021/Advanced%20Biochem/pcbi.1003329.pdf | ||
| + | #https://www.ncbi.nlm.nih.gov/books/NBK22572/#:~:text=Kinesins%20moving%20along%20microtubules%20usually,surfaces%20of%20some%20eukaryotic%20cells. | ||
| + | #https://www.ninds.nih.gov/Disorders/Patient-Caregiver-Education/Fact-Sheets/Charcot-Marie-Tooth-Disease-Fact-Sheet | ||
<references/> | <references/> | ||
Current revision
Kinesin Motor Domain
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References
- file:///C:/Users/Muna/Desktop/Spring%202021/Advanced%20Biochem/pcbi.1003329.pdf
- https://www.ncbi.nlm.nih.gov/books/NBK22572/#:~:text=Kinesins%20moving%20along%20microtubules%20usually,surfaces%20of%20some%20eukaryotic%20cells.
- https://www.ninds.nih.gov/Disorders/Patient-Caregiver-Education/Fact-Sheets/Charcot-Marie-Tooth-Disease-Fact-Sheet
