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| | ==Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei== | | ==Crystallographic structure of mutant C73S of thioredoxin from Litopenaeus vannamei== |
| - | <StructureSection load='5g31' size='340' side='right' caption='[[5g31]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5g31' size='340' side='right'caption='[[5g31]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5g31]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5G31 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5g31]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Penaeus_vannamei Penaeus vannamei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G31 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5g2z|5g2z]], [[5g30|5g30]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [https://pdbe.org/5g31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [https://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5g31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g31 OCA], [http://pdbe.org/5g31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5g31 RCSB], [http://www.ebi.ac.uk/pdbsum/5g31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5g31 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B1PWB9_PENVA B1PWB9_PENVA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thioredoxin-disulfide reductase]] | + | [[Category: Large Structures]] |
| - | [[Category: Campos-Acevedo, A A]] | + | [[Category: Penaeus vannamei]] |
| - | [[Category: Rudino-Pinera, E]] | + | [[Category: Campos-Acevedo AA]] |
| - | [[Category: Disulfide bond]] | + | [[Category: Rudino-Pinera E]] |
| - | [[Category: Litopenaeus vannamei]]
| + | |
| - | [[Category: Mutant]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Shrimp]]
| + | |
| - | [[Category: Thioredoxin]]
| + | |
| Structural highlights
Function
B1PWB9_PENVA
Publication Abstract from PubMed
The quaternary structure of the redox protein thioredoxin (Trx) has been debated. For bacterial Trx, there is no question regarding its monomeric state. In humans and other eukaryotes, the presence of a cysteine residue at the crystallographic symmetry axis points to the relevance of dimer formation in solution and in vivo. Crystallographic data for shrimp thioredoxin (LvTrx) obtained under different redox conditions reveal a dimeric arrangement mediated by a disulfide bond through residue Cys73 and other hydrophobic interactions located in the crystallographic interface, as reported for human Trx. Through the analysis of five mutants located at the crystallographic interface, this study provides structural and biochemical evidence for the existence in solution of monomeric and dimeric populations of wild-type LvTrx and five mutants. Based on the results of biochemical assays, SAXS studies and the crystallographic structures of three of the studied mutants (Cys73Ser, Asp60Ser and Trp31Ala), it is clear that the Cys73 residue is essential for dimerization. However, its mutation to Ser produces an enzyme which has similar redox activity in vitro to the wild type. A putative regulatory function of dimerization is proposed based on structural analysis. Nonetheless, the biological role of LvTrx dimerization needs to be experimentally unveiled. Additionally, the findings of this work reopen the discussion regarding the existence of similar behaviour in human thioredoxin, which shares a Cys at position 73 with LvTrx, a structural feature that is also present in some Trxs from vertebrates and crustaceans.
Is dimerization a common feature in thioredoxins? The case of thioredoxin from Litopenaeus vannamei.,Campos-Acevedo AA, Sotelo-Mundo RR, Perez J, Rudino-Pinera E Acta Crystallogr D Struct Biol. 2017 Apr 1;73(Pt 4):326-339. doi:, 10.1107/S2059798317002066. Epub 2017 Mar 31. PMID:28375144[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Campos-Acevedo AA, Sotelo-Mundo RR, Perez J, Rudino-Pinera E. Is dimerization a common feature in thioredoxins? The case of thioredoxin from Litopenaeus vannamei. Acta Crystallogr D Struct Biol. 2017 Apr 1;73(Pt 4):326-339. doi:, 10.1107/S2059798317002066. Epub 2017 Mar 31. PMID:28375144 doi:http://dx.doi.org/10.1107/S2059798317002066
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