5k04

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==The NatB Acetyltransferase Complex Bound To CoA and MES==
==The NatB Acetyltransferase Complex Bound To CoA and MES==
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<StructureSection load='5k04' size='340' side='right' caption='[[5k04]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='5k04' size='340' side='right'caption='[[5k04]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5k04]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K04 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5k04]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans_WO-1 Candida albicans WO-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K04 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5k18|5k18]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k04 OCA], [http://pdbe.org/5k04 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k04 RCSB], [http://www.ebi.ac.uk/pdbsum/5k04 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k04 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k04 OCA], [https://pdbe.org/5k04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k04 RCSB], [https://www.ebi.ac.uk/pdbsum/5k04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k04 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/C4YFL7_CANAW C4YFL7_CANAW]
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The NatB N-terminal acetyltransferase specifically acetylates the N-terminal group of substrate protein peptides starting with Met-Asp/Glu/Asn/Gln. How NatB recognizes and acetylates these substrates remains unknown. Here, we report crystal structures of a NatB holoenzyme from Candida albicans in the presence of its co-factor CoA and substrate peptides. The auxiliary subunit Naa25 of NatB forms a horseshoe-like deck to hold specifically its catalytic subunit Naa20. The first two amino acids Met and Asp of a substrate peptide mediate the major interactions with the active site in the Naa20 subunit. The hydrogen bonds between the substrate Asp and pocket residues of Naa20 are essential to determine the NatB substrate specificity. Moreover, a hydrogen bond between the amino group of the substrate Met and a carbonyl group in the Naa20 active site directly anchors the substrate toward acetyl-CoA. Together, these structures define a unique molecular mechanism of specific N-terminal acetylation acted by NatB.
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Molecular Basis of Substrate Specific Acetylation by N-Terminal Acetyltransferase NatB.,Hong H, Cai Y, Zhang S, Ding H, Wang H, Han A Structure. 2017 Apr 4;25(4):641-649.e3. doi: 10.1016/j.str.2017.03.003. PMID:28380339<ref>PMID:28380339</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5k04" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cai, Y]]
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[[Category: Candida albicans WO-1]]
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[[Category: Han, A]]
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[[Category: Large Structures]]
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[[Category: Hong, H]]
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[[Category: Cai Y]]
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[[Category: Zhang, S]]
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[[Category: Han A]]
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[[Category: N-terminal acetyltransferase complex]]
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[[Category: Hong H]]
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[[Category: Transferase]]
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[[Category: Zhang S]]

Current revision

The NatB Acetyltransferase Complex Bound To CoA and MES

PDB ID 5k04

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