4tw0

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of SCARB2 in Acidic Condition (pH4.8)

Structural highlights

4tw0 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.648Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

SCRB2_HUMAN Unverricht-Lundborg disease;Gaucher disease type 1;Action myoclonus - renal failure syndrome. The disease is caused by mutations affecting the gene represented in this entry. Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.

Function

SCRB2_HUMAN Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.^[1]

Publication Abstract from PubMed

Unlike the well-established picture for the entry of enveloped viruses, the mechanism of cellular entry of non-enveloped eukaryotic viruses remains largely mysterious. Picornaviruses are representative models for such viruses, and initiate this entry process by their functional receptors. Here we present the structural and functional studies of SCARB2, a functional receptor of the important human enterovirus 71 (EV71). SCARB2 is responsible for attachment as well as uncoating of EV71. Differences in the structures of SCARB2 under neutral and acidic conditions reveal that SCARB2 undergoes a pivotal pH-dependent conformational change which opens a lipid-transfer tunnel to mediate the expulsion of a hydrophobic pocket factor from the virion, a pre-requisite for uncoating. We have also identified the key residues essential for attachment to SCARB2, identifying the canyon region of EV71 as mediating the receptor interaction. Together these results provide a clear understanding of cellular attachment and initiation of uncoating for enteroviruses.

Molecular mechanism of SCARB2-mediated attachment and uncoating of EV71.,Dang M, Wang X, Wang Q, Wang Y, Lin J, Sun Y, Li X, Zhang L, Lou Z, Wang J, Rao Z Protein Cell. 2014 Sep;5(9):692-703. doi: 10.1007/s13238-014-0087-3. Epub 2014, Jul 2. PMID:24986489^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reczek D, Schwake M, Schroder J, Hughes H, Blanz J, Jin X, Brondyk W, Van Patten S, Edmunds T, Saftig P. LIMP-2 is a receptor for lysosomal mannose-6-phosphate-independent targeting of beta-glucocerebrosidase. Cell. 2007 Nov 16;131(4):770-83. PMID:18022370 doi:http://dx.doi.org/10.1016/j.cell.2007.10.018
↑ Dang M, Wang X, Wang Q, Wang Y, Lin J, Sun Y, Li X, Zhang L, Lou Z, Wang J, Rao Z. Molecular mechanism of SCARB2-mediated attachment and uncoating of EV71. Protein Cell. 2014 Sep;5(9):692-703. doi: 10.1007/s13238-014-0087-3. Epub 2014, Jul 2. PMID:24986489 doi:http://dx.doi.org/10.1007/s13238-014-0087-3

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tw0"

Categories: Homo sapiens | Large Structures | Dang MH | Rao ZH | Wang XX

@@ Line 1: / Line 1: @@
 ==Crystal Structure of SCARB2 in Acidic Condition (pH4.8)==
-<StructureSection load='4tw0' size='340' side='right' caption='[[4tw0]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
+<StructureSection load='4tw0' size='340' side='right'caption='[[4tw0]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4tw0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TW0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4tw0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TW0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.648&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tvz|4tvz]], [[4tw2|4tw2]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tw0 OCA], [http://pdbe.org/4tw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4tw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tw0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4tw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tw0 OCA], [https://pdbe.org/4tw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4tw0 RCSB], [https://www.ebi.ac.uk/pdbsum/4tw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4tw0 ProSAT]</span></td></tr>
 </table>
 == Disease ==
-[[http://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN]] Unverricht-Lundborg disease;Gaucher disease type 1;Action myoclonus - renal failure syndrome. The disease is caused by mutations affecting the gene represented in this entry.  Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
+[https://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN] Unverricht-Lundborg disease;Gaucher disease type 1;Action myoclonus - renal failure syndrome. The disease is caused by mutations affecting the gene represented in this entry.  Genetic variants in SCARB2 can act as modifier of the phenotypic expression and severity of Gaucher disease.
 == Function ==
-[[http://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN]] Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.<ref>PMID:18022370</ref>
+[https://www.uniprot.org/uniprot/SCRB2_HUMAN SCRB2_HUMAN] Acts as a lysosomal receptor for glucosylceramidase (GBA) targeting.<ref>PMID:18022370</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Dang, M H]]
+[[Category: Homo sapiens]]
-[[Category: Rao, Z H]]
+[[Category: Large Structures]]
-[[Category: Wang, X X]]
+[[Category: Dang MH]]
-[[Category: Lipid binding tunnel]]
+[[Category: Rao ZH]]
-[[Category: Protein binding]]
+[[Category: Wang XX]]

4tw0

From Proteopedia

Current revision

Crystal Structure of SCARB2 in Acidic Condition (pH4.8)

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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