1uxc

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[[Image:1uxc.gif|left|200px]]
 
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{{Structure
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==FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, MINIMIZED STRUCTURE==
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|PDB= 1uxc |SIZE=350|CAPTION= <scene name='initialview01'>1uxc</scene>
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<StructureSection load='1uxc' size='340' side='right'caption='[[1uxc]]' scene=''>
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|SITE= <scene name='pdbsite=HTH:Hth_laci+Family'>HTH</scene>
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1uxc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UXC FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxc OCA], [https://pdbe.org/1uxc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uxc RCSB], [https://www.ebi.ac.uk/pdbsum/1uxc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxc ProSAT]</span></td></tr>
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|DOMAIN=
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</table>
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|RELATEDENTRY=[[1uxd|1UXD]]
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxc OCA], [http://www.ebi.ac.uk/pdbsum/1uxc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uxc RCSB]</span>
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[https://www.uniprot.org/uniprot/CRA_ECOLI CRA_ECOLI] Global transcriptional regulator, which plays an important role in the regulation of carbon metabolism. Activates transcription of genes encoding biosynthetic and oxidative enzymes (involved in Krebs cycle, glyoxylate shunt and gluconeogenesis, such as ppsA and fbp). Represses genes involved in sugar catabolism, such as fruB, pfkA, pykF and adhE. Binds asymmetrically to the two half-sites of its operator.<ref>PMID:16115199</ref> <ref>PMID:8195118</ref> <ref>PMID:8230205</ref> <ref>PMID:8550429</ref> <ref>PMID:8577250</ref> <ref>PMID:8858581</ref> <ref>PMID:9084760</ref> <ref>PMID:9371462</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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'''FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, MINIMIZED STRUCTURE'''
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxc_consurf.spt"</scriptWhenChecked>
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==Overview==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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FruR is an Escherichia coli transcriptional regulator that belongs to the LacI DNA-binding protein family. By using 1H and 15N NMR spectroscopy, we have determined the three-dimensional solution structure of the FruR N-terminal DNA-binding domain consisting of 57 amino acid residues. A total of 809 NMR-derived distances and 54 dihedral angle constraints have been used for molecular modelling with the X-PLOR program. The resulting set of calculated structures presents an average root-mean-square deviation of 0.37 A at the main-chain level for the first 47 residues. This highly defined N-terminal part of the structure reveals a similar topology for the three alpha-helices when compared to the 3D structures of LacI and PurR counterparts. The most striking difference lies in the connection between helix II and helix III, in which three additional residues are present in FruR. This connecting segment is well structured and contains a type III turn. Apart from hydrophobic interactions of non-polar residues with the core of the domain, this connecting segment is stabilised by several hydrogen bonds and by the aromatic ring stacking between Tyr19 of helix II and Tyr28 of the turn. The region containing the putative "hinge helix" (helix IV), that has been described in PurR-DNA complex to make specific base contacts in the minor groove of DNA, is unfolded. Examination of hydrogen bonds highlights the importance of homologous residues that seem to be conserved for their ability to fulfill helix N and C-capping roles in the LacI repressor family.
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==About this Structure==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uxc ConSurf].
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1UXC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXC OCA].
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<div style="clear:both"></div>
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== References ==
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==Reference==
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<references/>
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Three-dimensional structure of the DNA-binding domain of the fructose repressor from Escherichia coli by 1H and 15N NMR., Penin F, Geourjon C, Montserret R, Bockmann A, Lesage A, Yang YS, Bonod-Bidaud C, Cortay JC, Negre D, Cozzone AJ, Deleage G, J Mol Biol. 1997 Jul 18;270(3):496-510. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9237914 9237914]
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__TOC__
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[[Category: Escherichia coli]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Escherichia coli K-12]]
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[[Category: Bockmann, A.]]
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[[Category: Large Structures]]
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[[Category: Bonod-Bidaud, C.]]
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[[Category: Bockmann A]]
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[[Category: Cortay, J C.]]
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[[Category: Bonod-Bidaud C]]
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[[Category: Cozzone, A J.]]
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[[Category: Cortay JC]]
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[[Category: Deleage, G.]]
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[[Category: Cozzone AJ]]
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[[Category: Geourjon, C.]]
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[[Category: Deleage G]]
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[[Category: Lesage, A.]]
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[[Category: Geourjon C]]
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[[Category: Montserret, R.]]
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[[Category: Lesage A]]
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[[Category: Negre, D.]]
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[[Category: Montserret R]]
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[[Category: Penin, F.]]
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[[Category: Negre D]]
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[[Category: Yang, Y.]]
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[[Category: Penin F]]
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[[Category: dna-binding protein]]
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[[Category: Yang Y]]
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[[Category: fructose repressor]]
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[[Category: laci family]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:39 2008''
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Current revision

FRUCTOSE REPRESSOR DNA-BINDING DOMAIN, NMR, MINIMIZED STRUCTURE

PDB ID 1uxc

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