5nro
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5nro is ON HOLD until Paper Publication Authors: Kopp, J., Kityk, R., Mayer, M.P. Description: Structure of a chaperone with bound ATP [[Category: ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of full-length DnaK with bound J-domain== | |
| + | <StructureSection load='5nro' size='340' side='right'caption='[[5nro]], [[Resolution|resolution]] 3.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5nro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NRO FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nro OCA], [https://pdbe.org/5nro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nro RCSB], [https://www.ebi.ac.uk/pdbsum/5nro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nro ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DNAK_ECOLI DNAK_ECOLI] Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.[HAMAP-Rule:MF_00332] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Efficient targeting of Hsp70 chaperones to substrate proteins depends on J-domain cochaperones, which in synergism with substrates trigger ATP hydrolysis in Hsp70s and concomitant substrate trapping. We present the crystal structure of the J-domain of Escherichia coli DnaJ in complex with the E. coli Hsp70 DnaK. The J-domain interacts not only with DnaK's nucleotide-binding domain (NBD) but also with its substrate-binding domain (SBD) and packs against the highly conserved interdomain linker. Mutational replacement of contacts between J-domain and SBD strongly reduces the ability of substrates to stimulate ATP hydrolysis in the presence of DnaJ and compromises viability at heat shock temperatures. Our data demonstrate that the J-domain and the substrate do not deliver completely independent signals for ATP hydrolysis, but the J-domain, in addition to its direct influence on Hsp70s catalytic center, makes Hsp70 more responsive for the hydrolysis-inducing signal of the substrate, resulting in efficient substrate trapping. | ||
| - | + | Molecular Mechanism of J-Domain-Triggered ATP Hydrolysis by Hsp70 Chaperones.,Kityk R, Kopp J, Mayer MP Mol Cell. 2017 Dec 27. pii: S1097-2765(17)30931-0. doi:, 10.1016/j.molcel.2017.12.003. PMID:29290615<ref>PMID:29290615</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Kityk | + | <div class="pdbe-citations 5nro" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kopp | + | |
| - | [[Category: Mayer | + | ==See Also== |
| + | *[[Heat Shock Protein structures|Heat Shock Protein structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kityk R]] | ||
| + | [[Category: Kopp J]] | ||
| + | [[Category: Mayer MP]] | ||
Current revision
Structure of full-length DnaK with bound J-domain
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