5vkv
From Proteopedia
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(New page: '''Unreleased structure''' The entry 5vkv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution NMR structure of the membrane electron transporter CcdA== | |
| + | <StructureSection load='5vkv' size='340' side='right'caption='[[5vkv]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5vkv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VKV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vkv OCA], [https://pdbe.org/5vkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vkv RCSB], [https://www.ebi.ac.uk/pdbsum/5vkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vkv ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5SIG0_THET8 Q5SIG0_THET8] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Membrane oxidoreductase CcdA plays a central role in supplying reducing equivalents from the bacterial cytoplasm to the envelope. It transports electrons across the membrane using a single pair of cysteines by a mechanism that has not yet been elucidated. Here we report an NMR structure of the Thermus thermophilus CcdA (TtCcdA) in an oxidized and outward-facing state. CcdA consists of two inverted structural repeats of three transmembrane helices (2 x 3-TM). We computationally modeled and experimentally validated an inward-facing state, which suggests that CcdA uses an elevator-type movement to shuttle the reactive cysteines across the membrane. CcdA belongs to the LysE superfamily, and thus its structure may be relevant to other LysE clan transporters. Structure comparisons of CcdA, semiSWEET, Pnu, and major facilitator superfamily (MFS) transporters provide insights into membrane transporter architecture and mechanism. | ||
| - | + | Solution structure and elevator mechanism of the membrane electron transporter CcdA.,Zhou Y, Bushweller JH Nat Struct Mol Biol. 2018 Feb;25(2):163-169. doi: 10.1038/s41594-018-0022-z. Epub, 2018 Jan 29. PMID:29379172<ref>PMID:29379172</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5vkv" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermus thermophilus HB8]] | ||
| + | [[Category: Bushweller JH]] | ||
| + | [[Category: Zhou Y]] | ||
Current revision
Solution NMR structure of the membrane electron transporter CcdA
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