4rl8

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==Crystal structure of the COG4313 outer membrane channel from Pseudomonas putida F1==
==Crystal structure of the COG4313 outer membrane channel from Pseudomonas putida F1==
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<StructureSection load='4rl8' size='340' side='right' caption='[[4rl8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='4rl8' size='340' side='right'caption='[[4rl8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4rl8]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RL8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4rl8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida_F1 Pseudomonas putida F1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RL8 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rl8 OCA], [http://pdbe.org/4rl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rl8 RCSB], [http://www.ebi.ac.uk/pdbsum/4rl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rl8 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rl8 OCA], [https://pdbe.org/4rl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rl8 RCSB], [https://www.ebi.ac.uk/pdbsum/4rl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rl8 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A5W3Z9_PSEP1 A5W3Z9_PSEP1]
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COG4313 proteins form a large and widespread family of outer membrane channels and have been implicated in the uptake of a variety of hydrophobic molecules. Structure-function studies of this protein family have so far been hampered by a lack of structural information. Here we present the X-ray crystal structure of Pput2725 from the biodegrader Pseudomonas putida F1, a COG4313 channel of unknown function, using data to 2.3 A resolution. The structure shows a 12-stranded barrel with an N-terminal segment preceding the first beta-strand occluding the lumen of the barrel. Single channel electrophysiology and liposome swelling experiments suggest that while the narrow channel visible in the crystal structure does allow passage of ions and certain small molecules in vitro, Pput2725 is unlikely to function as a channel for hydrophilic molecules. Instead, the presence of bound detergent molecules inside the barrel suggests that Pput2725 mediates uptake of hydrophobic molecules. Sequence alignments and the locations of highly conserved residues suggest the presence of a dynamic lateral opening through which hydrophobic molecules might gain entry into the cell. Our results provide the basis for structure-function studies of COG4313 family members with known function, such as the SphA sphingosine uptake channel of Pseudomonas aeruginosa.
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Crystal structure of a COG4313 outer membrane channel.,Berg Bv, Bhamidimarri SP, Winterhalter M Sci Rep. 2015 Jul 7;5:11927. doi: 10.1038/srep11927. PMID:26149193<ref>PMID:26149193</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4rl8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Berg, B van den]]
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[[Category: Large Structures]]
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[[Category: Bacterial outer membrane]]
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[[Category: Pseudomonas putida F1]]
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[[Category: Beta barrel]]
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[[Category: Van den Berg B]]
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[[Category: Channel]]
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[[Category: Membrane protein]]
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Current revision

Crystal structure of the COG4313 outer membrane channel from Pseudomonas putida F1

PDB ID 4rl8

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