5xj1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5xj1 is ON HOLD Authors: Jiang, Y., Gong, Q. Description: Crystal structure of spRlmCD Category: Unreleased Structures Category: Jiang, Y [...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of spRlmCD== | |
| + | <StructureSection load='5xj1' size='340' side='right'caption='[[5xj1]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XJ1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.753Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xj1 OCA], [https://pdbe.org/5xj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xj1 RCSB], [https://www.ebi.ac.uk/pdbsum/5xj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xj1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Y1029_STRPN Y1029_STRPN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RlmCD has recently been identified as the S-adenosyl methionine (SAM)-dependent methyltransferase responsible for the formation of m5U at U747 and U1939 of 23S ribosomal RNA in Streptococcus pneumoniae. In this research, we determine the high-resolution crystal structures of apo-form RlmCD and its complex with SAH. Using an in-vitro methyltransferase assay, we reveal the crucial residues for its catalytic functions. Furthermore, structural comparison between RlmCD and its structural homologue RumA, which only catalyzes the m5U1939 in Escherichia coli, implicates that a unique long linker in the central domain of RlmCD is the key factor in determining its substrate selectivity. Its significance in the enzyme activity of RlmCD is further confirmed by in-vitro methyltransferase assay. | ||
| - | + | Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD.,Jiang Y, Li F, Wu J, Shi Y, Gong Q PLoS One. 2017 Sep 26;12(9):e0185226. doi: 10.1371/journal.pone.0185226., eCollection 2017. PMID:28949991<ref>PMID:28949991</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5xj1" style="background-color:#fffaf0;"></div> |
| - | [[Category: Gong | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptococcus pneumoniae TIGR4]] | ||
| + | [[Category: Gong Q]] | ||
| + | [[Category: Jiang Y]] | ||
Current revision
Crystal structure of spRlmCD
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