5h2a

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==Crystal structure of Osh1 ANK domain from Kluyveromyces lactis==
==Crystal structure of Osh1 ANK domain from Kluyveromyces lactis==
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<StructureSection load='5h2a' size='340' side='right' caption='[[5h2a]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='5h2a' size='340' side='right'caption='[[5h2a]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5h2a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H2A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5h2a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H2A FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h2d|5h2d]], [[5h2c|5h2c]], [[5h2b|5h2b]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2a OCA], [http://pdbe.org/5h2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h2a RCSB], [http://www.ebi.ac.uk/pdbsum/5h2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2a ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h2a OCA], [https://pdbe.org/5h2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h2a RCSB], [https://www.ebi.ac.uk/pdbsum/5h2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h2a ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q6CUK7_KLULA Q6CUK7_KLULA]
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Yeast Osh1 belongs to the oxysterol-binding protein (OSBP) family of proteins and contains multiple targeting modules optimized for lipid transport at the nucleus-vacuole junction (NVJ). The key determinants for NVJ targeting and the role of Osh1 at NVJs have remained elusive because of unknown lipid specificities. In this study, we determined the structures of the ankyrin repeat domain (ANK), and OSBP-related domain (ORD) of Osh1, in complex with Nvj1 and ergosterol, respectively. The Osh1 ANK forms a unique bi-lobed structure that recognizes a cytosolic helical segment of Nvj1. We discovered that Osh1 ORD binds ergosterol and phosphatidylinositol 4-phosphate PI(4)P in a competitive manner, suggesting counter-transport function of the two lipids. Ergosterol is bound to the hydrophobic pocket in a head-down orientation, and the structure of the PI(4)P-binding site in Osh1 is well conserved. Our results suggest that Osh1 performs non-vesicular transport of ergosterol and PI(4)P at the NVJ.
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Structure of Yeast OSBP-Related Protein Osh1 Reveals Key Determinants for Lipid Transport and Protein Targeting at the Nucleus-Vacuole Junction.,Manik MK, Yang H, Tong J, Im YJ Structure. 2017 Apr 4;25(4):617-629.e3. doi: 10.1016/j.str.2017.02.010. Epub 2017, Mar 16. PMID:28319008<ref>PMID:28319008</ref>
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==See Also==
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*[[Oxysterol-binding protein homolog|Oxysterol-binding protein homolog]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5h2a" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Im, Y J]]
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[[Category: Kluyveromyces lactis NRRL Y-1140]]
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[[Category: Manik, M K]]
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[[Category: Large Structures]]
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[[Category: Tong, J S]]
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[[Category: Im YJ]]
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[[Category: Yang, H S]]
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[[Category: Manik MK]]
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[[Category: Ank nvj1]]
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[[Category: Tong JS]]
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[[Category: Lipid binding protein]]
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[[Category: Yang HS]]
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[[Category: Lipid transfer]]
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[[Category: Oxysterol binding]]
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Current revision

Crystal structure of Osh1 ANK domain from Kluyveromyces lactis

PDB ID 5h2a

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